Graphene doped Mn2 O3 Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer,Electroanalysis

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Graphene doped Mn2 O3 Nanofibers as a Facile Electroanalytical DNA Point Mutation Detection Platform for Early Diagnosis of Breast/Ovarian Cancer
Electroanalysis ( IF 2.7 ) Pub Date : 2018-06-07 , DOI: 10.1002/elan.201800220
Suryasnata Tripathy ₁ , Rahul Gangwar ₁ , Patta Supraja ₁ , AVSS Narayana Rao ₂ , Siva Rama Krishna Vanjari ₁ , Shiv Govind Singh ₁

Affiliation

This paper demonstrates a simple, label‐free detection methodology for detecting single point DNA mutations. Single point mutation detection is a key enabler for diagnosis and prevention of several genetic disorders that manifest into cancers. Specifically for this purpose, herein, an electrochemical biosensor utilizing electrospun graphene doped manganese III oxide nanofibers (GMnO) is developed. The charge transfer resistance offered by GMnO is extremely sensitive to the localized change in the conductivity. This sensitivity, attributed to the low band gap of Mn2O3 and high charge transfer kinetics of graphene, is explored in the proposed mutation detection platform. As a proof of concept, ultrasensitive detection of BRCA1 gene specific point mutation is demonstrated. The target specific single stranded probe DNA is immobilized onto GMnO modified glassy carbon working electrodes via chemisorption. Post target‐DNA hybridization, differential pulse voltammetry is employed to facilitate detection of targeted point mutation, wherein, difference in peak currents is used to distinguish the target DNA as normal or mutant. Efficiency of the proposed method is evaluated against a target concentration ranging from 10 pM−1 μM. With respect to the mutated target DNA, the LoD of the proposed device is found to be 0.8±0.069 pM. The proposed approach can be extended for detecting any mutation/hybridization of interest by simply adapting an appropriate functionalization protocol.

中文翻译：

石墨烯掺杂的 Mn2O3 纳米纤维作为一种简便的电分析 DNA 点突变检测平台，用于乳腺癌/卵巢癌的早期诊断

本文展示了一种用于检测单点 DNA 突变的简单、无标记的检测方法。单点突变检测是诊断和预防几种表现为癌症的遗传疾病的关键推动因素。特别是为此目的，本文开发了一种利用电纺石墨烯掺杂的三氧化三锰纳米纤维 (GMnO) 的电化学生物传感器。GMnO 提供的电荷转移电阻对电导率的局部变化极为敏感。这种敏感性归因于 Mn2O3 的低带隙和石墨烯的高电荷转移动力学，在所提出的突变检测平台中进行了探索。作为概念证明，展示了 BRCA1 基因特异性点突变的超灵敏检测。目标特异性单链探针 DNA 通过化学吸附固定在 GMnO 修饰的玻璃碳工作电极上。靶DNA杂交后，采用差分脉冲伏安法来促进靶点突变的检测，其中，峰值电流的差异用于区分靶DNA是正常的还是突变的。所提出方法的效率是针对 10 pM-1 μM 范围内的目标浓度进行评估的。对于突变的目标 DNA，发现所提出设备的 LoD 为 0.8±0.069 pM。通过简单地调整适当的功能化协议，可以扩展所提出的方法以检测任何感兴趣的突变/杂交。差分脉冲伏安法用于检测目标点突变，其中峰值电流的差异用于区分目标DNA是正常的还是突变的。针对 10 pM-1 μM 范围内的目标浓度评估所提出方法的效率。对于突变的目标 DNA，发现所提出设备的 LoD 为 0.8±0.069 pM。通过简单地调整适当的功能化协议，可以扩展所提出的方法以检测任何感兴趣的突变/杂交。差分脉冲伏安法用于检测目标点突变，其中峰值电流的差异用于区分目标DNA是正常的还是突变的。所提出方法的效率是针对 10 pM-1 μM 范围内的目标浓度进行评估的。对于突变的目标 DNA，发现所提出设备的 LoD 为 0.8±0.069 pM。通过简单地调整适当的功能化协议，可以扩展所提出的方法以检测任何感兴趣的突变/杂交。发现所提议设备的 LoD 为 0.8±0.069 pM。通过简单地调整适当的功能化协议，可以扩展所提出的方法以检测任何感兴趣的突变/杂交。发现所提议设备的 LoD 为 0.8±0.069 pM。通过简单地调整适当的功能化协议，可以扩展所提出的方法以检测任何感兴趣的突变/杂交。

更新日期：2018-06-07

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