Influence of the nature of the support on the catalytic performance of CALB: experimental and theoretical evidence,Catalysis Science & Technology

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Influence of the nature of the support on the catalytic performance of CALB: experimental and theoretical evidence
Catalysis Science & Technology ( IF 4.4 ) Pub Date : 2018-06-07 00:00:00 , DOI: 10.1039/c7cy02466e
Carla José _{1,

2,

3,

4,

5} , María Victoria Toledo _{1,

2,

3,

4,

5} , Paula Nicolás _{3,

5,

6,

7,

8} , Verónica Lasalle _{3,

5,

7,

9,

10} , María Luján Ferreira _{3,

5,

6,

7,

8} , Laura E. Briand _{1,

2,

3,

4,

5}

Affiliation

The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification of R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin, polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) exhibit higher conversion of ibuprofen and enantiomeric excess towards the S-enantiomer than CALB immobilized on hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless of the nature of the support material. A high enzyme loading is detrimental to the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidence for the influence of water activity in the stereospecificity of the lipase.

中文翻译：

载体性质对CALB催化性能的影响：实验和理论证据

南极假丝酵母（CALB）的脂肪酶B的固定化是通过吸附和共价键相互作用在各种亲水性和疏水性载体上进行的。在不添加助溶剂的情况下，R / S-布洛芬与乙醇的对映选择性酯化反应评估了生物催化剂的催化性能。本研究表明，基于CALB的生物催化剂通过吸附在疏水性载体（丙烯酸树脂，聚甲基丙烯酸甲酯，聚苯乙烯，环氧树脂和聚丙烯）上而固定化，布洛芬和对映异构体向S的转化率更高。-CALB-对映体比固定在亲水材料上。另外，酶的负载量是影响底物转化的关键因素，而与载体材料的性质无关。高的酶负载量不利于生物催化剂的比活性。实验和分子模型研究为水活度对脂肪酶立体定向性的影响提供了证据。

更新日期：2018-06-07

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