Six aflatoxins (AFs; AF B₁, B₂, G₁, G₂, M₁ and M₂) and six zearalenone (ZEN) analogs (ZEN, zearalanone, α-zeralanol, β-zeralanol, α-zearalenol, and β-zearalenol) were simultaneously extracted from edible and medicinal herbs using a group-specific immunoaffinity column (IAC) and then identified by ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS). The IAC was prepared by coupling N-hydroxysuccinimide-activated Sepharose 4B Fast Flow gel with two group-specific monoclonal antibodies. The column capacities to six AFs and six ZEN analogs ranged from 100.2 ng to 167.1 ng and from 59.5 ng to 244.4 ng, respectively. The IAC–UPLC–MS/MS method was developed and validated with three different matrices (Chinese yam [Dioscorea polystachya], Platycodon grandiflorum and coix seed [Semen Coicis]). Recoveries of twelve analytes from edible and medicinal herbs were in the range of 64.7%–112.1%, with relative standard deviations below 13.7%. The limits of quantification were in the range from 0.08 μg kg⁻¹ to 0.2 μg kg⁻¹. The method was proven to be sensitive and accurate, and suitable for the determination of real samples.

六种黄曲霉毒素（AFs; AF B ₁，B ₂，G ₁，G ₂，M ₁和M ₂）和六种玉米赤霉烯酮（ZEN）类似物（ZEN，泽拉酮，α-泽拉洛尔醇，β-泽拉洛尔，α-泽拉来醇和β -玉米赤霉烯醇）是使用组特异性免疫亲和柱（IAC）同时从食用和草药中提取的，然后通过超高效液相色谱串联质谱（UPLC-MS / MS）进行鉴定。IAC是通过耦合N来准备的-羟基琥珀酰亚胺活化的Sepharose 4B Fast Flow凝胶，带有两个组特异性单克隆抗体。六个AF和六个ZEN类似物的色谱柱容量分别为100.2 ng至167.1 ng和59.5 ng至244.4 ng。IAC–UPLC–MS / MS方法已开发并通过三种不同的基质（山药[ Dioscorea polystachya ]，桔梗和co种子[Semen Coicis]）进行了验证。食用和草药中十二种分析物的回收率在64.7％–112.1％的范围内，相对标准偏差低于13.7％。定量限是在范围从0.08微克千克^-1至0.2微克千克^-1。事实证明该方法灵敏，准确，适用于实际样品的测定。