Detecting and Imaging of γ-Glutamytranspeptidase Activity in Serum, Live Cells, and Pathological Tissues with a High Signal-Stability Probe by Releasing a Precipitating Fluorochrome,ACS Sensors

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Detecting and Imaging of γ-Glutamytranspeptidase Activity in Serum, Live Cells, and Pathological Tissues with a High Signal-Stability Probe by Releasing a Precipitating Fluorochrome
ACS Sensors ( IF 8.2 ) Pub Date : 2018-06-07 00:00:00 , DOI: 10.1021/acssensors.8b00274
Juan Ou-Yang ₁ , Yong-Fei Li _{1,

2} , Ping Wu ₁ , Wen-Li Jiang ₁ , Hong-Wen Liu ₁ , Chun-Yan Li _{1,

3}

Affiliation

γ-Glutamytranspeptidase (GGT) is a significant tumor-related biomarker that overexpresses in several tumor cells. Accurate detection and imaging of GGT activity in serum, live cells, and pathological tissues hold great significance for cancer diagnosis, treatment, and management. Recently developed small molecule fluorescent probes for GGT tend to diffuse to the whole cytoplasm and then translocate out of live cells after enzymatic reaction, which make them fail to provide high spatial resolution and long-term imaging in biological systems. To address these problems, a novel fluorescent probe (HPQ-PDG) which releases a precipitating fluorochrome upon the catalysis of GGT is designed and synthesized. HPQ-PDG is able to detect GGT activity with high spatial resolution and good signal-stability. The large Stokes shift of the probe enables it to detect the activity of GGT in serum samples with high sensitivity. To our delight, the probe is used for imaging GGT activity in live cells with the ability of discriminating cancer cells from normal cells. What’s more, we successfully apply it for pathological tissues imaging, with the results indicating that the potential application of HPQ-PDG in histopathological examination. All these results demonstrate the potential application of HPQ-PDG in the clinic.

中文翻译：

通过释放沉淀的荧光染料用高信号稳定性探针检测和成像血清，活细胞和病理组织中的γ-谷氨酰胺转肽酶活性

γ-谷氨酰胺转肽酶（GGT）是一种重要的肿瘤相关生物标志物，在某些肿瘤细胞中过表达。血清，活细胞和病理组织中GGT活性的准确检测和成像对癌症的诊断，治疗和管理具有重要意义。最近开发的用于GGT的小分子荧光探针倾向于扩散到整个细胞质，然后在酶促反应后从活细胞中转移出来，这使其无法在生物系统中提供高空间分辨率和长期成像。为了解决这些问题，设计并合成了新颖的荧光探针（HPQ-PDG），其在GGT催化下释放出沉淀的荧光染料。HPQ-PDG能够以高空间分辨率和良好的信号稳定性检测GGT活性。探针的大斯托克斯位移使其能够以高灵敏度检测血清样品中GGT的活性。令我们高兴的是，该探针用于对活细胞中的GGT活性进行成像，并具有区分癌细胞与正常细胞的能力。此外，我们成功地将其应用于病理组织成像，结果表明HPQ-PDG在组织病理学检查中的潜在应用。所有这些结果证明了HPQ-PDG在临床中的潜在应用。结果表明HPQ-PDG在组织病理学检查中的潜在应用。所有这些结果证明了HPQ-PDG在临床中的潜在应用。结果表明HPQ-PDG在组织病理学检查中的潜在应用。所有这些结果证明了HPQ-PDG在临床中的潜在应用。

更新日期：2018-06-07

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