The ability to measure the levels of diagnostically relevant proteins, such as antibodies, directly at the point of care could significantly impact healthcare. Thus motivated, we explore here the E-DNA “scaffold” sensing platform, a rapid, convenient, single-step means to this end. These sensors comprise a rigid nucleic acid “scaffold” attached via a flexible linker to an electrode and modified on its distal end with a redox reporter and a protein binding “recognition element”. The binding of a targeted protein reduces the efficiency with which the redox reporter approaches the electrode, resulting in an easily measured signal change when the sensor is interrogated voltammetrically. Previously we have demonstrated scaffold sensors employing a range of low molecular weight haptens and linear peptides as their recognition elements. Expanding on this here we have characterized sensors employing much larger recognition elements (up to and including full length proteins) in order to (1) define the range of recognition elements suitable for use in the platform; (2) better characterize the platform’s signaling mechanism to aid its design and optimization; and (3) demonstrate the analytical performance of sensors employing full-length proteins as recognition elements. In doing so we have enlarged the range of molecular targets amenable to this rapid and convenient sensing platform.

中文翻译：

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扩大蛋白质检测电化学DNA“脚手架”传感器的范围

直接在护理点测量诊断相关蛋白（例如抗体）水平的能力可能会严重影响医疗保健。因此，在此基础上，我们探索了E-DNA“支架”传感平台，这是一种快速，便捷，一步一步的方法。这些传感器包括一个通过柔性连接器连接到电极上的刚性核酸“支架”，并在其远端用氧化还原报告基因和结合蛋白质的“识别元件”进行修饰。靶蛋白质的结合降低了氧化还原报告基因接近电极的效率，当通过伏安法查询传感器时，导致容易测量的信号变化。以前，我们已经展示了采用一系列低分子量半抗原和线性肽作为其识别元件的支架传感器。在此扩展，我们对使用更大的识别元件（最多并包括全长蛋白质）的传感器进行了特征化，以便（1）定义适合在平台中使用的识别元件的范围；（2）更好地描述平台的信号机制，以帮助其设计和优化；（3）证明了使用全长蛋白质作为识别元件的传感器的分析性能。为此，我们扩大了适用于此快速便捷的传感平台的分子靶标范围。（2）更好地描述平台的信号机制，以帮助其设计和优化；（3）证明了使用全长蛋白质作为识别元件的传感器的分析性能。为此，我们扩大了适用于这种快速便捷的传感平台的分子靶标的范围。（2）更好地描述平台的信号机制，以帮助其设计和优化；（3）证明了使用全长蛋白质作为识别元件的传感器的分析性能。为此，我们扩大了适用于此快速便捷的传感平台的分子靶标范围。