Engineering Multifunctional Coatings on Nanoparticles Based on Oxidative Coupling Assembly of Polyphenols for Stimuli-Responsive Drug Delivery,Journal of Agricultural and Food Chemistry

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Engineering Multifunctional Coatings on Nanoparticles Based on Oxidative Coupling Assembly of Polyphenols for Stimuli-Responsive Drug Delivery
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-07 00:00:00 , DOI: 10.1021/acs.jafc.8b01208
Hongshan Liang _{1,

2} , Bin Zhou ₃ , Jing Li _{1,

2} , Xingnian Liu _{1,

2} , Ziyu Deng _{1,

2} , Bin Li _{1,

2,

4}

Affiliation

In this study, zein nanoparticles (NPs) with novel multifunctional coatings based on oxidative coupling assembly of polyphenols were synthesized for the first time. This coating was formed by oxidative self-polymerization of the organic ligands (polyphenols) in an alkaline condition, which could be biodegraded by acidic pH, as a result, impacting the pH-responsive property of the system. More importantly, the high level of intracellular glutathione (GSH) could induce the biodegradation of the polyphenol coatings, resulting in a fast release of trapped anticancer drugs in the cells. Based on confocal laser scanning microscopy (CLSM) and cytotoxicity experiments, drug-loaded and polyphenol-coated zein NPs were shown to possess highly efficient internalization and an apparent cytotoxic effect on HeLa cells. Notably, the CLSM observation illustrated that coated zein NPs showed delayed drug release compared with free drug or DOX-loaded zein NPs without coatings, resulting from the pH-responsive release of loaded drug in the extra/intracellular environment. Additionally, the short-time cytotoxicity and morphology observation also confirmed the delayed drug release behavior of coated NPs. These highly biocompatible and biodegradable polyphenol-coated zein NPs may be promising vectors in the field of controlled-release biomedical applications and cancer therapy.

中文翻译：

基于多酚氧化偶联组装的纳米颗粒工程多功能涂料用于刺激响应性药物的递送

在本研究中，首次合成了基于多酚氧化偶联组装的新型多功能玉米醇溶蛋白纳米粒（NPs）。该涂层是在碱性条件下通过有机配体（多酚）的氧化自聚合作用形成的，而该酸性条件可被酸性pH值生物降解，从而影响系统的pH响应特性。更重要的是，高水平的细胞内谷胱甘肽（GSH）可以诱导多酚涂层的生物降解，从而导致捕获的抗癌药物在细胞中快速释放。基于共聚焦激光扫描显微镜（CLSM）和细胞毒性实验，载药的和多酚涂层的玉米醇溶蛋白NPs具有高效的内在化作用，并且对HeLa细胞具有明显的细胞毒性作用。尤其，CLSM观察结果表明，包衣的玉米醇溶蛋白NPs与不含包衣的游离药物或DOX负载的玉米醇溶蛋白NPs相比，显示出药物释放延迟，这是由于在细胞外/细胞内环境中，pH响应释放了负载的药物。另外，短期的细胞毒性和形态学观察也证实了包被的NP的延迟的药物释放行为。这些高度生物相容性和可生物降解的多酚涂层玉米醇溶蛋白NP在控释生物医学应用和癌症治疗领域可能是有前途的载体。短时的细胞毒性和形态学观察也证实了包被的NP的延迟的药物释放行为。这些高度生物相容性和可生物降解的多酚涂层玉米醇溶蛋白NP在控释生物医学应用和癌症治疗领域可能是有前途的载体。短时的细胞毒性和形态学观察也证实了包被的NP的延迟的药物释放行为。这些高度生物相容性和可生物降解的多酚涂层玉米醇溶蛋白NP在控释生物医学应用和癌症治疗领域可能是有前途的载体。

更新日期：2018-06-07

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