Interactions between microorganisms and minerals have the potential contribution to remove polycyclic aromatic hydrocarbons (PAHs) in model systems. In this study, phenanthrene (PHE) was used as a probe molecule to explore the potential adsorption and biotransformation processes in the presence of microorganisms and various reference clays, such as montmorillonite (M), kaolinite (K), and pyrophyllite (P). Equilibrium adsorption experiments and scanning electron microscopy (SEM) technique were used to investigate the sorption of Pantoea agglomerans strains on clay minerals saturated with cations (Na⁺ and Fe³⁺). The adsorption isotherms of PHE and Pantoea agglomerans strains on cation-modified clay minerals fitted to Langmuir equation, and their adsorbed amounts both followed the sequence: montmorillonite > kaolinite > pyrophyllite. For six types of cation-modified minerals, the behavior of PHE adsorbed and Pantoea agglomerans adhered onto mentioned minerals was in the order of Na(I)-M > Fe(Ⅲ)-M, Na(I)-K > Fe(Ⅲ)-K and Fe(Ⅲ)-P > Na(I)-P, respectively. The biodegradation results showed that cation-modified clay minerals could enhance the biodegradation of PHE, ascribing to their large specific surface area, and cation exchange capability, as well as the difference in zeta potential between minerals and Pantoea agglomerans strains. Comparison of biodegradation rates displayed that PHE was degraded the highest in the presence of Na-M (93.285%). In addition, the obtained results suggested that the adhesion of bacteria onto cation-exchanged clay minerals was beneficial to the biodegradation of PHE. Anthracen-9-ylmethanol and 3,4-dimethyl-2-(3-methylbutanoyl)benzoic acid were detected as the main intermediate compounds, which can be further biodegraded into small molecules. The overall results obtained in this study are of valuable significance for the understanding of the behavior of PHE in soil and associated environment.

微生物与矿物质之间的相互作用可能对去除模型系统中的多环芳烃（PAHs）产生潜在的影响。在这项研究中，菲（PHE）被用作探针分子，以探索在存在微生物和各种参考粘土（如蒙脱土（M），高岭石（K）和叶蜡石（P））的情况下潜在的吸附和生物转化过程。利用平衡吸附实验和扫描电子显微镜（SEM）技术研究了Pantoea aglomerans菌株在阳离子（Na ⁺和Fe ³⁺）饱和的粘土矿物上的吸附。PHE和Pantoea团聚体的吸附等温线符合Langmuir方程的阳离子改性粘土矿物上的菌株，其吸附量均遵循以下顺序：蒙脱石>高岭石>叶蜡石。对于六种类型的阳离子改性矿物质，吸附的PHE的行为和附着在所述矿物质上的泛菌的排列顺序为Na （ I）-M> Fe（Ⅲ）-M，Na（I）-K> Fe（Ⅲ） ）-K和Fe（Ⅲ）-P> Na（I）-P。生物降解结果表明，阳离子改性粘土矿物具有较大的比表面积，阳离子交换能力以及矿物与泛菌之间的ζ电势差，因此可以促进PHE的生物降解。株。生物降解速率的比较显示，在Na-M存在下，PHE的降解最高（93.285％）。另外，获得的结果表明，细菌在阳离子交换的粘土矿物上的粘附有利于PHE的生物降解。检测到蒽-9-基甲醇和3,4-二甲基-2-（3-甲基丁酸）苯甲酸是主要的中间体化合物，可以将其进一步生物降解为小分子。这项研究中获得的总体结果对于了解PHE在土壤和相关环境中的行为具有重要的意义。