The livestock wastewater treatment plant represents an important reservoir of antibiotic resistance determinants in the environment. The study explored the prevalence of five antibiotic resistance genes (ARGs, including sulI, tetA, qnrD, mphB and mcr−1) and class 1 integron (intI1) in a typical livestock wastewater treatment plant, and analyzed their integrated association with two metal resistance genes (copA and czcA), two pathogens genes (Staphylococcus and Campylobacter), bacterial community and wastewater properties. Results indicated that all investigated genes were detected in the plant. The treatment plant could not completely remove ARGs abundances, with up to 2.2 × 10⁴~3.7 × 10⁸ copies/L of them remaining in the effluent. Mcr−1 was further enriched by 27-fold in the subsequent pond. The correlation analysis showed that mphB significantly correlateed with tetA and intI. Mcr−1 strongly correlated with copA. MphB and intI significantly correlated with czcA. The correlations implied a potential co-selection risk of bacterial resistant to antibiotics and metals. Redundancy analyses indicated that qnrD and mcr−1 strongly correlated with 13 and 14 bacterial genera, respectively. Most ARGs positively correlated to wastewater nutrients, indicating that an efficient reduction of wastewater nutrients would contribute to the antibiotic resistance control. The study will provide useful implications on fates and reductions of ARGs in livestock facilities and receiving environments.

牲畜废水处理厂是环境中抗生素抗性决定因素的重要储存库。这项研究探索了五个抗生素抗性基因（ARG，包括sul I，tet A，qnr D，mph B和mcr -1）和1类整合子（int I1）的流行情况，并分析了它们的整合与两个金属抗性基因（cop A和czc）相关A），两种病原体基因（葡萄球菌和弯曲杆菌），细菌群落和废水特性。结果表明在植物中检测到所有被调查的基因。的处理厂不能完全除去的ARG丰度，具有高达2.2×10 ⁴〜3.7×10 ⁸个拷贝/他们L的残留在流出物。在随后的池塘中，Mcr -1进一步富集了27倍。相关分析表明英里有显著correlateed乙TET A和INT一MCR -1强烈相关警察A. MPH B和诠释我有显著相关CZC答：相关性暗示细菌对抗生素和金属有抗性的潜在共选风险。冗余分析表明qnr D和mcr -1分别与13和14个细菌属密切相关。大多数ARG与废水中的养分呈正相关，表明有效减少废水中的养分有助于控制抗生素的耐药性。该研究将对牲畜设施和接收环境中的命运和降低ARGs提供有用的启示。