Terpyridine derivatives as “turn-on” fluorescence chemosensors for the selective and sensitive detection of Zn2+ ions in solution and in live cells†,Photochemical & Photobiological Sciences

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Terpyridine derivatives as “turn-on” fluorescence chemosensors for the selective and sensitive detection of Zn2+ ions in solution and in live cells†
Photochemical & Photobiological Sciences ( IF 2.7 ) Pub Date : 2018-06-06 00:00:00 , DOI: 10.1039/c8pp00186c
Tripti Mandal _{1,

2,

3,

4} , Anowar Hossain _{1,

2,

3,

4} , Anamika Dhara _{1,

2,

3,

4} , Abdulla Al Masum _{2,

3,

4,

5} , Saugata Konar _{1,

4,

6,

7} , Saikat Kumar Manna _{1,

4,

8,

9} , Saikat Kumar Seth _{2,

3,

4,

10} , Sudipta Pathak _{1,

4,

8,

9} , Subrata Mukhopadhyay _{1,

2,

3,

4}

Affiliation

A terpyridine based compound L1 was designed and synthesized as an “off–on” chemosensor for the detection of Zn²⁺. Chemosensor L1 showed excellent selectivity and sensitivity toward Zn²⁺ by exhibiting a large fluorescence enhancement (∼51-fold) at 370 nm whereas other competitive metal ions did not show any noticeable change in the emission spectra of chemosensor L1. The chemosensor (L1) was shown to detect Zn²⁺ ions down to 9.76 μM at pH 7.4. However, chemosensor L1 binds Zn²⁺ in a 1 : 2 ratio (receptor : metal) with an association constant of 1.85 × 10⁴ (R² = 0.993) and this 1 : 2 stoichiometric fashion is established on the basis of a Job plot and mass spectroscopy. DFT/TD-DFT calculations were carried out to understand the binding nature, coordination features and electronic properties of L1 and the L1–2Zn²⁺ complex. In addition, this “turn-on” fluorescence probe was effectively used to image intracellular Zn²⁺ ions in cultured MDA-MB-468 cells.

中文翻译：

特吡啶吡啶衍生物作为“开启”荧光化学传感器，用于选择性和灵敏地检测溶液和活细胞中的Zn ²⁺离子†

设计并合成了一种基于联吡啶的化合物L1作为“开-关”化学传感器，用于检测Zn ²⁺。化学传感器L1通过在370 nm处表现出较大的荧光增强（〜51倍），表现出对Zn ²⁺的优异选择性和敏感性，而其他竞争性金属离子在化学传感器L1的发射光谱中未显示任何明显变化。化学传感器（L1）在pH 7.4时可检测到低至9.76μM的Zn ²⁺离子。但是，化学传感器L1以1：1的比率（受体：金属）结合Zn ²⁺，缔合常数为1.85×10 ⁴（R² = 0.993），并且这种1：2的化学计量方式是基于Job图和质谱法建立的。DFT / TD-DFT计算进行了理解的约束性，协调功能和电子特性L1和L1 -2Zn²⁺复合物。此外，这种“开启”荧光探针可有效地用于成像培养的MDA-MB-468细胞中的细胞内Zn²⁺离子。

更新日期：2018-06-06

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