Cutleaf groundcherry (Physalis angulata L.) is an annual plant with a number of medicinal ingredients. However, studies about the secondary metabolism of P. angulata are very limited. An integrated metabolome and proteome approach was used to reveal the variations in the metabolism associated with bioactive compounds under methyl-jasmonate (MeJA) treatment. Application of MeJA to the hairy roots could significantly increase the accumulation of most active ingredients. A targeted approach confirmed the variations in physalins D and H between MeJA treatment and the controls. Increases in the levels of a number of terpenoid backbone biosynthesis and steroid biosynthesis related enzymes, cytochrome P450 monooxygenases and 3β-hydroxysterioid dehydrogenase might provide a potential explanation for the MeJA-induced active ingredient synthesis. Our results may contribute to a deeper understanding of the regulation mechanism underlying the MeJA-induced active compound accumulation in P. angulata.

中文翻译：

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代谢组学和蛋白质组学的比较分析揭示了MeJA诱导的刺叶地毛（Physalis angulata L.）毛根中潜在的生物活性化合物积累的调控机制。

Cutleaf地面樱桃（Physalis angulata L.）是一年生植物，含有多种药用成分。然而，有关红毛。次生代谢的研究非常有限。集成的代谢组学和蛋白质组学方法用于揭示茉莉酸甲酯（MeJA）处理下与生物活性化合物相关的代谢变化。在毛状根上施用MeJA可以显着增加大多数活性成分的积累。一种有针对性的方法证实了MeJA处理和对照之间physalins D和H的变化。大量萜类生物骨架生物合成和类固醇生物合成相关酶，细胞色素P450单加氧酶和3β-羟基类固醇脱氢酶水平的增加可能为MeJA诱导的活性成分合成提供了潜在的解释。我们的结果可能有助于更深入地了解MeJA诱导的活性化合物积累的调控机制。P. angulata。