The seven-spotted ladybird beetle, Coccinella septempunctata L., as a dominant predator of aphids, has played a crucial role in integrated pest management (IPM) strategies in agricultural ecosystems. To study the risk of insecticides to C. septempunctata, the neonicotinoid clothianidin was selected for evaluation of its influence on C. septempunctata at lethal and sublethal doses. The LR₅₀ (application rate causing 50% mortality) in the exposed larvae decreased from 19.94 to 5.91 g a.i. ha⁻¹, and the daily HQ (hazard quotient) values increased from 3.00 to 10.15, indicating potential intoxication risks. We also determined NOERs (No Observed Effect application Rates) of clothianidin on the total developmental time (10 g a.i. ha⁻¹), survival (2.5 g a.i. ha⁻¹) and pupation (5 g a.i. ha⁻¹). Moreover, clothianidin at a NOER of 2.5 g a.i. ha⁻¹ did not profoundly affect adult emergence, fecundity or egg hatchability. The total effect (E) assessment also showed that clothianidin at 2.5 g a.i. ha⁻¹ was slightly harmful to C. septempunctata. These results suggested that clothianidin would impair C. septempunctata when applied at over 2.5 g a.i. ha⁻¹ in the field. Conservation of this biological control agent in agricultural ecosystems thus requires further measures to decrease the applied dosages of clothianidin.

七斑瓢虫瓢虫Coccinella septempunctata L.是蚜虫的主要捕食者，在农业生态系统的病虫害综合防治（IPM）策略中发挥了关键作用。为了研究杀虫剂对七叶念珠菌的风险，选择了新烟碱类可尼丁以评估其在致死和半致死剂量下对七叶念珠菌的影响。暴露幼虫的LR ₅₀（导致50％死亡率的施用率）从19.94 g ai ha ^-1降低到5.91 g ai ha ^-1，并且每日HQ（危险商）值从3.00增加到10.15，表明存在潜在的中毒风险。我们还确定了可尼丁苷对总发育时间（10 g ai ha ^-1），存活率（2.5 g ai ha ^-1）和化脓（5 g ai ha ^-1）的NOER（无观察到的施用率）。此外，可可尼丁的NOER为2.5 g ai ha ^-1并没有深刻影响成年后的成年，繁殖力或卵孵化率。总体效果（E）评估还显示，2.5 g ai ha ^-1的可比尼丁对C. septempunctata有害。这些结果表明，当以超过2.5 g ai ha的量施用时，可比尼丁会损害C. septempunctata^-1在现场。因此，在农业生态系统中保护这种生物防治剂需要采取进一步措施，以减少可尼丁的使用剂量。