Attenuation of tert-Butyl Hydroperoxide (t-BHP)-Induced Oxidative Damage in HepG2 Cells by Tangeretin: Relevance of the Nrf2–ARE and MAPK Signaling Pathways,Journal of Agricultural and Food Chemistry

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Attenuation of tert-Butyl Hydroperoxide (t-BHP)-Induced Oxidative Damage in HepG2 Cells by Tangeretin: Relevance of the Nrf2–ARE and MAPK Signaling Pathways
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-06 00:00:00 , DOI: 10.1021/acs.jafc.8b01875
Fuqiang Liang ₁ , Yajing Fang ₁ , Weiwei Cao ₁ , Zhuo Zhang ₁ , Siyi Pan ₁ , Xiaoyun Xu ₁

Affiliation

The current study evaluates the protective effects of tangeretin, a representative polymethoxyflavone (PMF) mainly isolated from the peels of citrus fruits, against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells and the potential mechanisms of this protection. Tangeretin suppressed t-BHP-induced oxidative damage, as evaluated by cell viability, reactive-oxygen-species (ROS) levels, lactate dehydrogenase (LDH) leakage and glutathione (GSH) levels. Further mechanistic studies showed that tangeretin up-regulated the expression of heme oxygenase 1 (HO-1) and NAD(P)H quinone oxidoreductase 1 (NQO1). Moreover, tangeretin induced antioxidant-responsive-element (ARE)-dependent luciferase activation, nuclear factor (erythroid-derived 2)-like 2 (Nrf2) nuclear translocation, and mitogen-activated-protein-kinase (MAPK) phosphorylation. Results in the study indicate that the protective effects of tangeretin may be at least partly due to its capacity to up-regulate the antioxidant enzymes NQO1 and HO-1 via the MAPK–Nrf2–ARE signaling pathway. Tangeretin may play an effective protective role in liver injury.

中文翻译：

桔皮素对叔丁基过氧化氢（t -BHP）诱导的HepG2细胞氧化损伤的减弱：Nrf2-ARE和MAPK信号通路的相关性

目前的研究评估了橘皮苷（一种主要从柑橘类果皮中分离出来的代表性聚甲氧基黄酮（PMF））对叔丁基过氧化氢（t- BHP）诱导的HepG2细胞氧化损伤的保护作用以及这种保护作用的潜在机制。橘子抑制t-BHP诱导的氧化损伤，通过细胞活力，活性氧（ROS）水平，乳酸脱氢酶（LDH）泄漏和谷胱甘肽（GSH）水平进行评估。进一步的机理研究表明，橘红素上调了血红素加氧酶1（HO-1）和NAD（P）H醌氧化还原酶1（NQO1）的表达。此外，橘皮苷诱导抗氧化剂响应元件（ARE）依赖的萤光素酶激活，核因子（类胡萝卜素衍生的2）样2（Nrf2）核易位以及促分裂原激活的蛋白激酶（MAPK）磷酸化。研究结果表明，橘皮苷的保护作用至少部分是由于其通过MAPK–Nrf2–ARE信号通路上调抗氧化酶NQO1和HO-1的能力。橘皮素可能在肝损伤中起有效的保护作用。

更新日期：2018-06-06

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