RhBMP-2 and concomitant rapid material degradation synergistically promote bone repair and regeneration with collagen–hydroxyapatite nanocomposites,Journal of Materials Chemistry B

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RhBMP-2 and concomitant rapid material degradation synergistically promote bone repair and regeneration with collagen–hydroxyapatite nanocomposites
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2018-06-05 00:00:00 , DOI: 10.1039/c8tb00405f
Wen Su _{1,

2,

3,

4} , Xiaomin Ma _{1,

2,

3,

4} , Zhe Sun _{1,

2,

3,

4} , Zeng Yi _{1,

2,

3,

4} , Xinxing Cui _{1,

2,

3,

4} , Guangcan Chen _{1,

2,

3,

4} , Xiangyu Chen _{1,

2,

3,

4} , Bo Guo _{2,

4,

5,

6,

7} , Xudong Li _{1,

2,

3,

4}

Affiliation

The effective treatment of bone defects is still a great challenge in clinical practice. Synthetic bone-grafting substitutes of composition and structure analogous to bone as well as incorporated with growth factors are considered to be a promising solution. In this study, a collagen–hydroxyapatite (CHA) nanocomposite scaffold was developed by collagen self-assembly with simultaneous HA synthesis. The physicochemical properties such as morphology, inorganic phase, thermal decomposition, specific surface area and pore size distribution were characterized. The osteogenicity of CHA in the absence or presence of recombinant human bone morphogenetic protein-2 (rhBMP-2) was assessed both by cell culturing and animal implantation experiments. The gene expression results showed that the osteogenic differentiation capacity of rat bone mesenchymal stem cells (rBMSCs) has been enhanced both by CHA and rhBMP-2. The efficient bone regeneration of femoral defects in rabbits was achieved with CHA and CHA pre-absorbed rhBMP-2 (CHA/B), confirmed by micro-computed tomography measurements, histological observation and immunohistochemical analyses. The CHA nanocomposite was completely degraded within 8 weeks and replaced by new bone. It was found that rhBMP-2 not only accelerated and enhanced bone formation, but also expedited the degradation of CHA. It is believed that the rhBMP-2 and concomitant rapid material degradation synergistically promote bone repair and regeneration with CHA. The biodegradation behavior of CHA in the presence of rhBMP-2 can be further investigated to gain an in-depth understanding of the complex interplays among biomaterials, growth factors and their target cells. The relevant knowledge will facilitate the search for a reasonable, safe and efficient methodology for the introduction of growth factors to biomaterials so as to achieve satisfactory tissue regeneration.

中文翻译：

RhBMP-2及其伴随的快速材料降解与胶原-羟基磷灰石纳米复合材料协同促进骨修复和再生

有效治疗骨缺损仍然是临床实践中的巨大挑战。具有与骨骼类似的成分和结构以及与生长因子结合的合成骨移植替代物被认为是一种有前途的解决方案。在这项研究中，胶原蛋白-羟基磷灰石（CHA）纳米复合材料支架是通过胶原蛋白自组装同时进行HA合成而开发的。表征了其理化性质，如形态，无机相，热分解，比表面积和孔径分布。通过细胞培养和动物植入实验评估了在不存在或存在重组人骨形态发生蛋白2（rhBMP-2）的情况下CHA的成骨性。基因表达结果表明，CHA和rhBMP-2均可增强大鼠骨间充质干细胞（rBMSCs）的成骨分化能力。CHA和CHA预吸收的rhBMP-2（CHA / B）可实现兔股骨缺损的有效骨再生，这已通过微计算机断层扫描测量，组织学观察和免疫组化分析得到了证实。CHA纳米复合材料在8周内完全降解，并被新骨替代。发现rhBMP-2不仅加速和增强了骨形成，而且加速了CHA的降解。据信，rhBMP-2及其伴随的快速物质降解协同促进了CHA的骨修复和再生。可以进一步研究在rhBMP-2存在下CHA的生物降解行为，以深入了解生物材料，生长因子及其靶细胞之间的复杂相互作用。相关知识将有助于寻找一种合理，安全和有效的方法，以将生长因子引入生物材料，从而实现令人满意的组织再生。

更新日期：2018-06-05

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