Transcriptomic Profiling Suggests That Promysalin Alters the Metabolic Flux, Motility, and Iron Regulation in Pseudomonas putida KT2440,ACS Infectious Diseases

当前位置： X-MOL 学术 › ACS Infect. Dis. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Transcriptomic Profiling Suggests That Promysalin Alters the Metabolic Flux, Motility, and Iron Regulation in Pseudomonas putida KT2440
ACS Infectious Diseases ( IF 4.0 ) Pub Date : 2018-05-25 00:00:00 , DOI: 10.1021/acsinfecdis.8b00041
Krista M. Giglio ₁ , Colleen E. Keohane ₂ , Paul V. Stodghill ₁ , Andrew D. Steele ₂ , Christian Fetzer ₃ , Stephan A. Sieber ₃ , Melanie J. Filiatrault _{1,

4} , William M. Wuest _{2,

5}

Affiliation

Promysalin, a secondary metabolite produced by P. putida RW10S1, is a narrow-spectrum antibiotic that targets P. aeruginosa over other Pseudomonas spp. P. putida KT2440, a nonproducing strain, displays increased swarming motility and decreased pyoverdine production in the presence of exogenous promysalin. Herein, proteomic and transcriptomic experiments were used to provide insight about how promysalin elicits responses in PPKT2440 and rationalize its species selectivity. RNA-sequencing results suggest that promysalin affects PPKT2440 by (1) increasing swarming in a flagella-independent manner; (2) causing cells to behave as if they were experiencing an iron-deficient environment, and (3) shifting metabolism away from glucose conversion to pyruvate via the Entner–Doudoroff pathway. These findings highlight nature’s ability to develop small molecules with specific targets, resulting in exquisite selectivity.

中文翻译：

转录组分析表明，早孕素改变了恶臭假单胞菌KT2440的代谢通量，运动性和铁调节。

Promysalin是恶臭假单胞菌RW10S1产生的次生代谢产物，是一种窄谱抗生素，比其他假单胞菌更能靶向铜绿假单胞菌。恶臭假单胞菌KT2440是一种非生产型菌株，在存在外源性早幼髓质素的情况下，显示出成群的运动能力增加，而嘧啶的产生减少。在这里，蛋白质组学和转录组学实验被用来提供关于早幼霉素在PPKT2440中如何引起应答和合理化其物种选择性的见解。RNA测序结果表明，早霉菌素通过以下方式影响PPKT2440：（1）以鞭毛非依赖性方式增加种群。（2）使细胞表现出好像在缺铁的环境中一样的行为；（3）通过Entner–Doudoroff途径使新陈代谢从葡萄糖转化为丙酮酸。这些发现突出了自然界开发具有特定靶标的小分子的能力，从而导致了精湛的选择性。

更新日期：2018-05-25

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11