In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation,Accounts of Chemical Research

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In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation
Accounts of Chemical Research ( IF 16.4 ) Pub Date : 2018-06-05 00:00:00 , DOI: 10.1021/acs.accounts.8b00147
Enrico Luchinat _{1,

2} , Lucia Banci _{1,

3}

Affiliation

Cellular structural biology methods are needed to characterize biological processes at atomic resolution in the physiological environment of the cell. Toward this goal, solution in-cell NMR is a powerful approach because it provides structural and dynamic data on macromolecules inside living cells. Several approaches have been developed for in-cell NMR in cultured human cells, which are needed to study processes related to human diseases that rely on the delivery of exogenous macromolecules to the cells. Such strategies, however, may not be applicable to proteins that are sensitive to the external environment or prone to aggregate and can introduce artifacts during protein purification or delivery.

中文翻译：

人细胞内的细胞内NMR：直接蛋白质表达可进行蛋白质折叠和成熟的结构研究

需要细胞结构生物学方法来以原子分辨率表征细胞生理环境中的生物学过程。为了实现这一目标，溶液内核磁共振技术是一种强大的方法，因为它提供了有关活细胞内部大分子的结构和动态数据。已经开发了几种用于在培养的人类细胞中进行细胞内NMR的方法，用于研究与人类疾病有关的过程，这些过程依赖于外源大分子向细胞的递送。然而，这样的策略可能不适用于对外部环境敏感或易于聚集并且可能在蛋白质纯化或递送过程中引入伪像的蛋白质。

更新日期：2018-06-05

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