Effects of the Physicochemical, Colloidal, and Biological Characteristics of Different Polymer Structures between α-Poly(l-lysine) and ε-Poly(l-lysine) on Polymeric Gene Delivery,Biomacromolecules

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Effects of the Physicochemical, Colloidal, and Biological Characteristics of Different Polymer Structures between α-Poly(l-lysine) and ε-Poly(l-lysine) on Polymeric Gene Delivery
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-06-04 00:00:00 , DOI: 10.1021/acs.biomac.8b00097
Kyoungnam Kim ₁ , Kitae Ryu ₁ , Yeon Su Choi ₁ , Yong-Yeon Cho ₁ , Joo Young Lee ₁ , Hye Suk Lee ₁ , Han Chang Kang ₁

Affiliation

Though α-poly(l-lysine) (APL) has been well-studied in gene delivery, ε-poly(l-lysine) (EPL) with same repeating unit of l-lysine but different structure has been rarely investigated. This study compared various effects of their different structures in gene delivery processes. EPL showed less cytotoxicity and more proton buffering capacity for endosomal release than APL. Also, EPL/pDNA polyplexes represented higher nucleus preference than APL/pDNA polyplexes. However, EPL had weaker affinities with pDNA than APL, leading to formation of larger EPL/pDNA complexes with less compactness and successively faster decomplexation. The resultant difference of their pDNA binding affinity caused lower cellular uptake and lower transfection efficiency of EPL/pDNA complexes than APL/pDNA complexes. Thus, this study confirmed that various effects of gene delivery processes are changed by chemical structure of polymeric gene carriers. Especially, despite the low transfection efficiency of EPL-based polyplexes, the study found potentials of EPL in cytocompatibility, endosomal release, and nuclear import.

中文翻译：

α-聚（l-赖氨酸）和ε-聚（l-赖氨酸）之间不同聚合物结构的理化，胶体和生物学特性对聚合基因传递的影响

尽管已经很好地研究了α-聚（l-赖氨酸）（APL）在基因传递中的作用，但ε-聚（l-赖氨酸）（EPL）具有相同的l重复单元-赖氨酸，但很少研究不同的结构。这项研究比较了它们不同结构在基因传递过程中的各种作用。与APL相比，EPL显示出更少的细胞毒性和更多的质子内吞释放质子缓冲能力。同样，EPL / pDNA复合物比APL / pDNA复合物具有更高的核优先性。但是，EPL与pDNA的亲和力比APL弱，导致形成更大的EPL / pDNA复合物，但紧密度较低，并随后分解速度更快。它们的pDNA结合亲和力的差异导致了细胞吸收和EPL / pDNA复合物的转染效率低于APL / pDNA复合物。因此，这项研究证实，基因传递过程的各种作用被聚合基因载体的化学结构所改变。尤其，

更新日期：2018-06-04

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