Visual Assay of Glutathione in Vegetables and Fruits Using Quantum Dot Ratiometric Hybrid Probes,Journal of Agricultural and Food Chemistry

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Visual Assay of Glutathione in Vegetables and Fruits Using Quantum Dot Ratiometric Hybrid Probes
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-06-04 00:00:00 , DOI: 10.1021/acs.jafc.8b00662
Aimin Chen _{1,

2} , Xiao Peng ₁ , Zaifa Pan ₁ , Kang Shao ₁ , Jing Wang ₁ , Maohong Fan _{2,

3}

Affiliation

Future food safety monitoring with simple, fast, and visual methods has become increasingly important. Accordingly, this work was designed to construct a new-style dual-emission ratiometric fluorescent probe ([email protected]₂@CdTe) for visual assay of glutathione (GSH) with a “turn on” strategy. After adding Hg²⁺, the red fluorescence of the outer CdTe quantum dots (QDs) was quenched through both electron transfer and ion-binding processes. Upon the addition of GSH, the red fluorescence occurred again owing to the strong affinity between GSH and Hg²⁺, whereas the inner green fluorescence of CdSe QDs was unchanged, leading to a clearly recognizable fluorescence color change (from green to orange–red). In the concentration range from 0.1 to 10 μM, the relative fluorescence intensity ratios (I₆₁₉/I₅₃₅) showed an excellent linear correlation with the concentration of GSH, and the detection limit was as low as 42 nM under optimal conditions. Meanwhile, the ratiometric hybrid probes were successfully applied for direct visual sensing GSH in real vegetable and fruit samples.

中文翻译：

量子点比例混合探针用于蔬菜和水果中谷胱甘肽的可视化测定

未来用简单，快速和可视化方法进行食品安全监控已变得越来越重要。因此，这项工作旨在构建一种新型的双发射比例荧光探针（[电子邮件保护] ₂ @CdTe），用于通过“开启”策略对谷胱甘肽（GSH）进行视觉检测。加入Hg ^2+后，外部CdTe量子点（QDs）的红色荧光通过电子转移和离子结合过程猝灭。加入GSH后，由于GSH与Hg ²⁺之间的强亲和力，红色荧光再次出现，而CdSe量子点的内部绿色荧光没有改变，导致明显可识别的荧光颜色变化（从绿色到橙红色）。在0.1至10μM的浓度范围内，相对荧光强度比（I ₆₁₉ / I ₅₃₅）与GSH浓度呈极好的线性关系，并且在最佳条件下的检出限低至42 nM。同时，比例混合探针已成功应用于真实蔬菜和水果样品中的直接视觉传感GSH。

更新日期：2018-06-04

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