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New Insights into the Mechanism of Uphill Excitation Energy Transfer from Core Antenna to Reaction Center in Purple Photosynthetic Bacteria
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2018-06-04 00:00:00 , DOI: 10.1021/acs.jpclett.8b01197 Li-Ming Tan 1 , Jie Yu 1 , Tomoaki Kawakami 2 , Masayuki Kobayashi 3 , Peng Wang 1 , Zheng-Yu Wang-Otomo 2 , Jian-Ping Zhang 1
The Journal of Physical Chemistry Letters ( IF 4.8 ) Pub Date : 2018-06-04 00:00:00 , DOI: 10.1021/acs.jpclett.8b01197 Li-Ming Tan 1 , Jie Yu 1 , Tomoaki Kawakami 2 , Masayuki Kobayashi 3 , Peng Wang 1 , Zheng-Yu Wang-Otomo 2 , Jian-Ping Zhang 1
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The uphill excitation energy transfer (EET) from the core antenna (LH1) to the reaction center (RC) of purple photosynthetic bacteria was investigated at room temperature by comparing the native LH1-RC from Thermochromatium (Tch.) tepidum with the hybrid LH1-RC from a mutant strain of Rhodobacter (Rba.) sphaeroides. The latter protein with chimeric Tch-LH1 and Rba-RC exhibits a substantially larger RC-to-LH1 energy difference (ΔE = 630 cm–1) of 3-fold thermal energy (3kBT). The spectroscopic and kinetics results are discussed on the basis of the newly reported high-resolution structures of Tch. tepidum LH1-RC, which allow us to propose the existence of a passage formed by LH1 BChls that facilitates the LH1 → RC EET. The semilogarithmic plot of the EET rate against ΔE was found to be linear over a broad range of ΔE, which consolidates the mechanism of thermal activation as promoted by the spectral overlap between the LH1 fluorescence and the special pair absorption of RC.
中文翻译：
紫色光合细菌上层激发能量从核心天线转移到反应中心的机理的新见解
从核心天线（LH1）上坡激发能量转移（EET）到紫色光合细菌的反应中心（RC）是由来自比较天然LH1-RC在室温下研究Thermochromatium（Tch的。）绿硫菌来源与混合LH1- RC的球形球形红细菌（Rba。）的突变株。后者具有Tch -LH1和Rba -RC嵌合蛋白的蛋白的RC到LH1的能量差（ΔE = 630 cm –1）大得多，是3倍热能（3 k B T）。在新近报道的Tch高分辨率结构的基础上讨论了光谱和动力学结果。tepidum LH1-RC，这使我们可以提出由LH1 BChls形成的通道的存在，该通道有利于LH1→RC EET。发现EET速率相对于ΔE的半对数图在较宽的ΔE范围内是线性的，这巩固了LH1荧光与RC的特殊对吸收之间的光谱重叠所促进的热激活机制。
更新日期：2018-06-04
中文翻译：
紫色光合细菌上层激发能量从核心天线转移到反应中心的机理的新见解
从核心天线（LH1）上坡激发能量转移（EET）到紫色光合细菌的反应中心（RC）是由来自比较天然LH1-RC在室温下研究Thermochromatium（Tch的。）绿硫菌来源与混合LH1- RC的球形球形红细菌（Rba。）的突变株。后者具有Tch -LH1和Rba -RC嵌合蛋白的蛋白的RC到LH1的能量差（ΔE = 630 cm –1）大得多，是3倍热能（3 k B T）。在新近报道的Tch高分辨率结构的基础上讨论了光谱和动力学结果。tepidum LH1-RC，这使我们可以提出由LH1 BChls形成的通道的存在，该通道有利于LH1→RC EET。发现EET速率相对于ΔE的半对数图在较宽的ΔE范围内是线性的，这巩固了LH1荧光与RC的特殊对吸收之间的光谱重叠所促进的热激活机制。
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