In this study, a new method for extracting and isolating squalene from pumpkin seed oil was developed using magnetic nanoparticles (MNPs). The inhibition effect of extracted squalene on angiogenesis was investigated in chick chorioallantoic membrane (CAM). Squalene extraction from pumpkin seed oil was performed in two steps: fatty acids (FAs) separation via hydrothermal adsorption on the magnetic iron oxide nanoparticles and squalene extraction using solvent extraction method at different temperatures. In the first step, the neural network modeling was applied to predict the optimum range of operating parameters for achieving squalene with the highest purity. Results of transmission electron microscopy (TEM) analysis showed that under optimum conditions, the average diameter of the MNPs coated with FAs was about 20 nm. In the second step, the purity of squalene was estimated at 93% with a separation recovery percentage of 83.24% at the optimum temperature of 25 °C to prevent squalene oxidation. The inhibition effect of extracted squalene on angiogenesis in CAM suggested that squalene extracted from pumpkin seed oil had a significant effect on the inhibition of angiogenesis in the chicken embryo chorioallantoic membrane. Finally, this process yielded two valuable products, i.e. squalene with high-purity and MNPs coated with FAs.

在这项研究中，开发了一种使用磁性纳米粒子（MNP）从南瓜籽油中提取和分离角鲨烯的新方法。在鸡绒膜尿囊膜（CAM）中研究了角鲨烯提取物对血管生成的抑制作用。从南瓜籽油中角鲨烯的提取分两个步骤进行：通过水热吸附在磁性氧化铁纳米颗粒上，在不同温度下采用溶剂萃取法提取角鲨烯。第一步，应用神经网络建模来预测最佳操作参数范围，以实现具有最高纯度的角鲨烯。透射电子显微镜（TEM）分析的结果表明，在最佳条件下，涂有FA的MNP的平均直径约为20 nm。在第二步中，估计角鲨烯的纯度为93％，在25°C的最佳温度下，防止角鲨烯氧化的分离回收率为83.24％。角鲨烯提取物对CAM中血管生成的抑制作用表明，从南瓜籽油中提取的角鲨烯对鸡胚绒膜尿囊膜中的血管生成具有明显的抑制作用。最后，此过程产生了两个有价值的产品，即高纯度角鲨烯和涂有FA的MNP。