Protein Colorimetry Experiments That Incorporate Intentional Discrepancies and Historical Narratives,Journal of Chemical Education

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Protein Colorimetry Experiments That Incorporate Intentional Discrepancies and Historical Narratives
Journal of Chemical Education ( IF 2.5 ) Pub Date : 2018-06-04 00:00:00 , DOI: 10.1021/acs.jchemed.7b00633
Nathan S. Astrof ₁ , Gail Horowitz ₂

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A set of biochemistry experiments based on the technique of protein colorimetry is described. Students used the Biuret and Bradford assays to qualitatively and quantitatively assess solutions for protein. Two unique features of these experiments are (1) the use of intentional discrepancies within each experiment to promote critical thinking and (2) the use of authentic historical narratives to introduce critical concepts in the interpretation of assays and demonstrate the relevance of laboratory work to “real-world” scenarios. Details on the implementation and the evaluation of these experiments are discussed.

中文翻译：

结合了意向差异和历史叙述的蛋白质比色法实验

描述了一组基于蛋白质比色技术的生化实验。学生使用Biuret和Bradford分析法定性和定量评估蛋白质溶液。这些实验的两个独特特征是：（1）在每个实验中使用故意的差异来促进批判性思维；（2）使用真实的历史叙述在分析方法的解释中引入批判性概念，并证明实验室工作与“现实”场景。讨论了有关这些实验的实施和评估的详细信息。

更新日期：2018-06-04

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