This study examined modulation effects of cell-free supernatants of two commonly studied probiotic bacteria Lactobacillus rhamnosus GG (LGG) and Escherichia coli Nissle 1917 (EcN) on mucin and cytokine profiles of human intestinal epithelial HT29-MTX cells. It was found that LGG and EcN supernatants differentially modulated MUC5AC and MUC5B mRNA and protein, and total mucin-like glycoprotein secretion. Regarding modulation of cytokine profiles, LGG supernatants moderately influenced the secretion of anti-inflammatory cytokines such as interleukin (IL)-4, IL-5, and IL-10, while those of EcN exerted a broad proinflammatory effect to intestinal epithelial cells by inducing the secretion of proinflammatory cytokines such as IL-8, monocyte chemotactic protein-1, transforming growth factor α, tumor necrosis factor α, granulocyte macrophage colony-stimulating factor, and interferon γ. These results suggested that LGG and EcN might produce different bioactive products that display differential modulation of mucin and cytokines, which may contribute to intestinal health and/or defense against bacteria/pathogens. PRACTICAL APPLICATION The results suggested that LGG and EcN might produce different bioactive products that display differential modulation of mucin and cytokines, which may contribute to intestinal health and/or defense against bacteria/pathogens.

中文翻译：

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鼠李糖乳杆菌 GG 和大肠杆菌 Nissle 1917 无细胞上清液对人肠上皮 HT29-MTX 细胞粘蛋白和细胞因子分泌的调节作用

本研究检查了两种常用益生菌鼠李糖乳杆菌 GG (LGG) 和大肠杆菌 Nissle 1917 (EcN) 的无细胞上清液对人肠上皮 HT29-MTX 细胞粘蛋白和细胞因子谱的调节作用。发现 LGG 和 EcN 上清液差异调节 MUC5AC 和 MUC5B mRNA 和蛋白，以及总粘蛋白样糖蛋白分泌。关于细胞因子谱的调节，LGG 上清液适度影响抗炎细胞因子如白细胞介素 (IL)-4、IL-5 和 IL-10 的分泌，而 EcN 的那些通过诱导肠上皮细胞对肠上皮细胞发挥广泛的促炎作用。促炎细胞因子如 IL-8、单核细胞趋化蛋白 1、转化生长因子 α、肿瘤坏死因子 α 的分泌，粒细胞巨噬细胞集落刺激因子和干扰素γ。这些结果表明，LGG 和 EcN 可能产生不同的生物活性产物，这些产物表现出对粘蛋白和细胞因子的不同调节，这可能有助于肠道健康和/或防御细菌/病原体。实际应用 结果表明，LGG 和 EcN 可能产生不同的生物活性产物，显示出对粘蛋白和细胞因子的不同调节，这可能有助于肠道健康和/或防御细菌/病原体。