In tissue engineering there is growing interest in fabricating highly engineered platforms designed to instruct cells towards the synthesis of tissues that reproduce their natural counterpart. In this context, a fundamental factor to take into account is the control over the final tissue orientation, especially for what concerns the replication of load-bearing tissues whose functions are strictly related to their microstructural organization. Starting from this point, in this work we have engineered a gelatin-based hydrogel in order to be patterned by 2-photon polymerization (2PP) lithography for the fabrication of instructive free standing building blocks designed to produce anisotropic collagen-based μtissues. Biological results clearly highlighted the strong relationship between μtissue orientation and such topographies, which resulted in a crucial element in the production of highly anisotropic μtissues.

中文翻译：

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通过使用基于明胶的构建基块来控制细胞合成的细胞外基质的方向†

在组织工程中，越来越需要制造高度工程化的平台，该平台旨在指导细胞合成能够复制其天然对应物的组织。在这种情况下，要考虑的基本因素是对最终组织方向的控制，尤其是对于涉及功能与它们的微结构组织严格相关的承重组织的复制而言。从这一点出发，在这项工作中，我们设计了一种基于明胶的水凝胶，以便通过2-光子聚合（2PP）光刻进行图案化，以制造具有指导意义的独立式结构单元，以生产各向异性的基于胶原的μ组织。生物学结果清楚地说明了组织定向与此类地形之间的密切关系，