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Effect of Cd on growth, physiological response, Cd subcellular distribution and chemical forms of Koelreuteria paniculata
Ecotoxicology and Environmental Safety ( IF 6.2 ) Pub Date : 2018-05-18 , DOI: 10.1016/j.ecoenv.2018.05.026
Lan Peng Yang , Jian Zhu , Ping Wang , Jing Zeng , Rong Tan , Yu Zhong Yang , Zhi Ming Liu

Koelreuteria paniculata were cultivated in nutrient solution with different concentrations of Cd (0, 50, 150, 250 and 500 µM) and sampled after 90 days. The resistance, translocation, accumulation and stress responses in Koelreuteria paniculata were investigated by hydroponic experiments. The results showed that Koelreuteria paniculata is an efficient Cd excluder that can tolerate high concentrations of Cd (up to 150–250 µM of Cd). The concentration of Cd never exceeds 5 ppm in leaves and 10 ppm in roots. The high concentration of Cd (≥ 250 µM) had a toxic effect on K. paniculata and significantly restricted the plant growth. The accumulation ability of Cd by different plant tissues followed the sequence of roots > leaves > stems. The bioconcentration factors and translocation factors both were less than 1. Cd has the highest content in the cell wall and is migrated to soluble fractions and organelles at high concentrations. Undissolved Cd phosphate, pectates and protein-bound Cd were the predominant forms. The low concentration of Cd (≤150 µM) promoted the synthesis of soluble proteins, AsA and GSH, while high concentration of Cd clearly inhibited the physiological and biochemical process, caused membrane lipid peroxidation and severe membrane damages, and increased MDA and H2O2 contents. POD, CAT and SOD exhibited positive and effective responses to low concentration Cd stress, but could not remove the toxicity caused by high concentration Cd stress. The content of IAA, GA and ZT decreased and ABA content was significantly increased under high-concentration Cd stress.



中文翻译:

镉对小叶锦鸡儿生长,生理响应,镉亚细胞分布和化学形态的影响

在浓度为Cd(0、50、150、250和500 µM)的营养液中培养Koelreuteria paniculata,并在90天后取样。通过水培试验研究了拟南芥(Koelreuteria paniculata)的抗性,易位,积累和胁迫响应。结果表明,Koelreuteria paniculata是一种有效的Cd排除剂,可以耐受高浓度的Cd(最高150-250 µM Cd)。镉在叶子中的浓度永远不会超过5 ppm,在根中的浓度永远不会超过10 ppm。高浓度的镉(≥250 µM)对潘氏克鲁维酵母具有毒性作用并严重限制了植物的生长。不同植物组织对Cd的积累能力遵循根>叶>茎的顺序。生物浓缩因子和转运因子均小于1。Cd在细胞壁中含量最高,并以高浓度迁移至可溶性级分和细胞器。主要的形式是未溶解的磷酸Cd,果胶和与蛋白质结合的Cd。低浓度的Cd(≤150µM)促进了可溶性蛋白质AsA和GSH的合成,而高浓度的Cd明显抑制了生理和生化过程,引起膜脂质过氧化和严重的膜损伤,并增加了MDA和H 2 O 2个内容。POD,CAT和SOD对低浓度Cd胁迫表现出积极有效的反应,但不能消除高浓度Cd胁迫引起的毒性。高浓度镉胁迫下,IAA,GA和ZT含量降低,ABA含量显着提高。

更新日期:2018-05-18
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