In the present study, to discover new biomarker of Asian freshwater clam (Corbicula fluminea) to assess impact of environmental pollutions, cholecystokinin (CCK), conopressin, and Neuropeptide FF (FFamide) in C. fluminea were selected as potent biomarkers. Therefore, their full-length cDNAs were cloned and characterized to investigate the molecular characteristics and expression patterns of neuropeptides in C. fluminea. According to the sequence analysis, CCK, conopressin, and FFamide encoded proteins of 173, 152, and 90 amino acids, respectively. Moreover, the multiple sequence alignment revealed that the bioactive regions of these neuropeptides were well conserved among different invertebrates. In addition, under basal conditions, CCK, conopressin and FFamide mRNA were mainly expressed in the visceral mass, whereas the FFamide mRNA was rarely detected in the foot and mantle. Exposure to 20 and 200 μg/L Tris (2-butoxyethyl) phosphate (TBOEP) and tri-butyl-phosphate (TBP) exposure significantly up-regulated the expression of the CCK and FFamide mRNAs in the visceral mass (p < 0.05), whereas no significant changes in conopressin mRNA levels were observed in response to any treatment. Therefore, CCK and FFamide of C. fluminea neuropeptides are feasible new biomarkers for screening and assessing responses to organophosphate chemicals.

在本研究中，为发现亚洲淡水蛤（（Corbicula fluminea）的新生物标记物以评估环境污染的影响，选择了C. fluminea中的胆囊收缩素（CCK），conopressin和Neuropeptide FF（FFamide）作为有效的生物标记物。因此，克隆并鉴定了它们的全长cDNA，以研究流感嗜血杆菌中神经肽的分子特征和表达模式。。根据序列分析，CCK，conopressin和FFamide分别编码173、152和90个氨基酸的蛋白质。此外，多序列比对揭示了这些神经肽的生物活性区域在不同的无脊椎动物之间是非常保守的。此外，在基础条件下，CCK，conopressin和FFamide mRNA主要在内脏包块中表达，而FFamide mRNA很少在脚和地幔中检测到。暴露于20和200μg/ L磷酸三（2-丁氧基乙基）酯（TBOEP）和磷酸三丁酯（TBP）暴露显着上调了内脏肿块中CCK和FFamide mRNA的表达（p <0.05），而对任何处理均未观察到降压药mRNA的显着变化。因此，C. fluminea的CCK和FFamide 神经肽是可行的新型生物标志物，用于筛选和评估对有机磷酸酯化学物质的反应。