当前位置：
X-MOL 学术
›
ACS Med. Chem. Lett.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Azithromycin-Induced Changes to Bacterial Membrane Properties Monitored in Vitro by Second-Harmonic Light Scattering
ACS Medicinal Chemistry Letters ( IF 3.5 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1021/acsmedchemlett.7b00499 Mohammad Sharifian Gh. 1 , Michael J. Wilhelm 1 , Hai-Lung Dai 1
ACS Medicinal Chemistry Letters ( IF 3.5 ) Pub Date : 2018-03-09 00:00:00 , DOI: 10.1021/acsmedchemlett.7b00499 Mohammad Sharifian Gh. 1 , Michael J. Wilhelm 1 , Hai-Lung Dai 1
Affiliation
|
We present a nonlinear light scattering method for monitoring, with real-time resolution and membrane specificity, changes in molecular adsorption, and transport at bacterial membranes induced by an antimicrobial compound. Specifically, time-resolved second-harmonic light scattering (SHS) is used to quantify azithromycin-induced changes to bacterial membrane permeability in colloidal suspensions of living Escherichia coli. Variations in membrane properties are monitored through changes in the adsorption and transport rates of malachite green, a hydrophobic cation that gives SHS signal. Regardless of concentration, instantaneous treatment with azithromycin showed no significant changes in membrane permeability. However, 1 h pretreatment with subminimum inhibitory concentrations of azithromycin induced an order-of-magnitude enhancement in the permeability of both the outer membrane and, through facilitation of a new transport mechanism, the cytoplasmic membrane of the bacteria as well. This study illustrates SHS as a novel tool for monitoring antimicrobial-induced changes to membrane properties in living bacteria.
中文翻译：
阿奇霉素诱导的二次谐波光散射体外监测的细菌膜特性变化。
我们提出了一种非线性光散射方法，用于监测实时分辨率和膜特异性，分子吸附的变化以及由抗菌化合物诱导的细菌膜运输。具体而言，时间分辨二次谐波光散射（SHS）用于量化阿奇霉素诱导的活大肠杆菌胶体悬液中细菌膜通透性的变化。膜性质的变化通过孔雀石绿（一种提供SHS信号的疏水性阳离子）的吸附和传输速率的变化进行监测。无论浓度如何，阿奇霉素的瞬时处理均未显示出膜通透性的显着变化。但是，用最低抑制浓度的阿奇霉素预处理1 h会导致外膜的通透性增强一个数量级，并且通过促进新的转运机制，细菌的细胞质膜也会增强。这项研究表明，SHS是监测抗菌剂引起的活细菌膜特性变化的一种新型工具。
更新日期：2018-03-09
中文翻译：
阿奇霉素诱导的二次谐波光散射体外监测的细菌膜特性变化。
我们提出了一种非线性光散射方法，用于监测实时分辨率和膜特异性，分子吸附的变化以及由抗菌化合物诱导的细菌膜运输。具体而言，时间分辨二次谐波光散射（SHS）用于量化阿奇霉素诱导的活大肠杆菌胶体悬液中细菌膜通透性的变化。膜性质的变化通过孔雀石绿（一种提供SHS信号的疏水性阳离子）的吸附和传输速率的变化进行监测。无论浓度如何，阿奇霉素的瞬时处理均未显示出膜通透性的显着变化。但是，用最低抑制浓度的阿奇霉素预处理1 h会导致外膜的通透性增强一个数量级，并且通过促进新的转运机制，细菌的细胞质膜也会增强。这项研究表明，SHS是监测抗菌剂引起的活细菌膜特性变化的一种新型工具。
京公网安备 11010802027423号