Proteins represent a versatile biopolymer material for the preparation of nanoparticles due to their biocompatibility, biodegradability, and low immunogenicity. This study presents a protein‐based nanoparticle system consisting of high surface PEGylated lysozyme polyethylene glycol‐modified lysozyme (LYZ_mPEG). This protein modification leads to a solubility switch, which allows a nanoparticle preparation using a mild double emulsion method without the need of surfactants. The method allows the encapsulation of large hydrophilic payloads inside of the protein‐based nanoparticle system. Native lysozyme (LYZ) was chosen as payload because of its innate activity as natural antibiotic. The mild particle preparation procedure retains the structure and activity of the enzyme which was successfully tested against the gram‐positive bacteria strain M. Luteus. In comparison, the particle system shows no toxicity to human cells. This first report of a full protein‐based particle material for the transport of large hydrophilic payloads opens up new therapeutic applications for biopolymer‐based delivery systems.

中文翻译：

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基于蛋白质的纳米颗粒，可输送具有抗菌活性的酶

蛋白质由于其生物相容性，生物可降解性和低免疫原性而代表了一种用于制备纳米颗粒的通用生物聚合物材料。这项研究提出了一种基于蛋白质的纳米粒子系统，该系统由高表面聚乙二醇化溶菌酶，聚乙二醇修饰的溶菌酶（LYZ _{mPEG）组成}）。这种蛋白质修饰导致溶解度转换，从而允许使用温和的双重乳液方法制备纳米颗粒，而无需使用表面活性剂。该方法可以将大型亲水性有效载荷包裹在基于蛋白质的纳米颗粒系统内部。选择天然溶菌酶（LYZ）作为有效载荷是因为它具有天然抗生素的先天活性。温和的颗粒制备程序保留了酶的结构和活性，该酶已成功针对革兰氏阳性细菌菌株M. Luteus进行了测试。相比之下，粒子系统对人体细胞没有毒性。第一份关于基于蛋白质的全颗粒材料用于大型亲水性有效载荷运输的报告，为基于生物聚合物的输送系统开辟了新的治疗应用领域。