In situ generation of photoactivatable aggregation-induced emission probes for organelle-specific imaging†,Chemical Science

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In situ generation of photoactivatable aggregation-induced emission probes for organelle-specific imaging†
Chemical Science ( IF 7.6 ) Pub Date : 2018-06-01 00:00:00 , DOI: 10.1039/c8sc01887a
Shiwu Li _{1,

2} , Xia Ling ₁ , Yuhan Lin ₁ , Anjun Qin ₁ , Meng Gao ₃ , Ben Zhong Tang _{1,

4}

Affiliation

Photoactivatable fluorescent probes are ideal tools for organelle study with a significant advantage of high spatiotemporal resolution. However, conventional photo-caged fluorophores for organelle-specific imaging suffer from several drawbacks, such as aggregation-caused quenching (ACQ), instability under ambient light, low photoactivation efficiency, and toxic photo-cleavage byproducts. Herein, we propose a strategy for in situ generation of photoactivatable aggregation-induced emission (AIE) probes of 2-(2-hydroxyphenyl)-benzothiazolines from easily available disulfide and thiol substrates through tandem S–S bond reduction and intramolecular cyclization reaction. Because the photoactivatable AIE probes can be in situ generated in a quantitative yield, they can be directly used for bio-imaging without complicated separation steps. Under both one- and NIR two-photon irradiation, excellent spatiotemporal resolution and high photoactivation efficiency were achieved for specific imaging of lipid droplets and lysosomes, respectively. Based on their in situ generation and adjustable organelle-targeting ability, the photoactivatable AIE probes could become an easy-to-use imaging tool in the study of the biological functions of organelles.

中文翻译：

用于细胞器特异性成像的光活化聚集诱导发射探针的原位生成†

光激活荧光探针是细胞器研究的理想工具，具有高时空分辨率的显着优势。然而，用于细胞器特异性成像的传统光笼荧光团存在几个缺点，例如聚集引起的猝灭 (ACQ)、环境光下的不稳定性、低光活化效率和有毒的光裂解副产物。在此，我们提出了一种通过串联 S-S 键还原和分子内环化反应从容易获得的二硫化物和硫醇底物原位生成 2-(2-羟基苯基)-苯并噻唑啉的光活化聚集诱导发射 (AIE) 探针的策略。因为可光活化的 AIE 探针可以原位它们以定量产率产生，无需复杂的分离步骤即可直接用于生物成像。在单光子和近红外双光子照射下，脂滴和溶酶体的特异性成像分别实现了出色的时空分辨率和高光活化效率。基于其原位生成和可调节的细胞器靶向能力，可光活化AIE探针可以成为研究细胞器生物学功能的一种易于使用的成像工具。

更新日期：2018-06-01

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