AbstractThe importance of monodisperse poly(ethylene glycols) (PEGs) and their derivatives is rapidly increasing. Focusing on the merits of heterobifunctional PEGs, we developed a chromatography-free procedure for the preparation of monodisperse monotosylated PEGs. This technique enables the preparation of 8- to 16-mers in relatively large quantities, with good yields and purities, and facilitates the investigation of PEG-based functional molecules. For example, we synthesized structured PEGs consisting of monodisperse PEGs and polyols, which had specific two-dimensional shapes. These structured PEGs exhibited unique properties in aqueous environments; the triangular molecule dehydrated at a lower temperature than linear compounds with a similar molecular weight and could thus suppress the aggregation of proteins at high temperatures. We also found that the introduction of an aromatic group into the PEG skeleton lowered the dehydration temperature, which decreased the critical solution temperatures (LCSTs). Recent achievements in the development of engineered poly(ethylene glycol)s (PEGs) will be highlighted in this review.A chromatography-free procedure for the preparation of monodisperse monotosylated PEGs allowed for the preparation of 8- to 16-mers in relatively large quantities in good yields and purities and facilitates the investigation of PEG-based functional molecules such as structured PEGs, which had specific two-dimensional shapes. For example, the triangular molecule dehydrated at a lower temperature than linear compounds with a similar molecular weight and can suppress aggregation of proteins at high temperatures. It was also found that the introduction of an aromatic group in the PEG skeleton lowered the dehydration temperature.

中文翻译：

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用于生物相关应用的单分散工程 PEG

摘要单分散聚（乙二醇）（PEG）及其衍生物的重要性正在迅速增加。着眼于异双功能 PEG 的优点，我们开发了一种无需色谱的程序，用于制备单分散单甲苯基化 PEG。该技术可以相对大量地制备 8 到 16 聚体，具有良好的产率和纯度，并有助于研究基于 PEG 的功能分子。例如，我们合成了由具有特定二维形状的单分散 PEG 和多元醇组成的结构化 PEG。这些结构化的 PEG 在水性环境中表现出独特的特性；三角形分子在比具有相似分子量的线性化合物更低的温度下脱水，因此可以抑制蛋白质在高温下的聚集。我们还发现，将芳香基团引入 PEG 骨架降低了脱水温度，从而降低了临界溶液温度 (LCST)。本综述将重点介绍工程聚（乙二醇）（PEG）开发的最新成就。 用于制备单分散单甲苯基化 PEG 的无色谱程序允许制备相对大量的 8 至 16 聚体具有良好的产率和纯度，并有助于研究基于 PEG 的功能分子，例如具有特定二维形状的结构化 PEG。例如，三角形分子在比具有相似分子量的线性化合物更低的温度下脱水并且可以在高温下抑制蛋白质的聚集。