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Retrospective analyses of archive phytotoxicity test data can help in assessing internal dynamics and stability of growth in laboratory duckweed cultures
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2018-05-28 , DOI: 10.1016/j.aquatox.2018.05.022
Viktor Oláh , Anna Hepp , Norma Yolanda Gaibor Vaca , Marianna Tamás , Ilona Mészáros

High growth potential of duckweed species (Lemnaceae family) has been utilized in wide range of research and practical applications. Based on literature data, however, it can be assumed that duckweed populations maintain constant growth rates only when short periods are considered but can vary over longer time scales. This intrinsic instability in growth can affect the interpretation of growth data. Duckweed phytotoxicity tests are usually performed according to highly standardized protocols. Therefore the archive data provide an opportunity for retrospective comparisons. In the present study we collected growth (frond number- and frond area-based relative growth rates) and morphology (average frond and colony sizes) data from control treatments of phytotoxicity tests. All the analyzed tests were carried out with the same Spirodela polyrhiza (L.) Schleid. (giant duckweed) clone (RDSC ID No. 5501) under the same experimental conditions over more than four years. We aimed to assess the overall variability of the above parameters and to test if intrinsic growth patterns affect growth data in short-term. In general, the results reflected high stability of the measured parameters in long term but also indicated that some temporal variability is inevitable which can bias the comparability of growth tests. The frond area-based relative growth rate resulted in smaller coefficient of variation than the usually preferred frond number-based one. The results also revealed a negative correlation between mean growth rates and their coefficients of variation. Therefore, it would be advisable to introduce higher minimal growth rates and/or maximized tolerable coefficients of variation for control cultures into the standard duckweed growth inhibition tests. Analyses of growth data aggregated on seasonal basis indicated faster growth and larger mean frond size in laboratory duckweed cultures from mid-autumn till mid-spring than during summer and early autumn. But, in shorter term (∼50 days) we did not observe distinct trends in growth suggesting that the successive frond generations have no effect on growth traits within this time-scale. Our results point to the importance of assessing intrinsic growth dynamics in duckweed cultures and also to the re-usability of the already collected phytotoxicity data in addressing new research questions.



中文翻译:

存档植物毒性测试数据的回顾性分析可以帮助评估实验室浮萍培养物的内部动力学和生长稳定性

浮萍物种(Lemnaceae家族)的高生长潜力已被广泛用于研究和实际应用中。但是,基于文献数据,可以假定浮萍种群仅在考虑到短期的情况下才能保持恒定的增长率,但可以在较长的时间范围内变化。增长的这种内在不稳定性可能会影响增长数据的解释。浮萍的植物毒性试验通常根据高度标准化的规程进行。因此,档案数据为回顾性比较提供了机会。在本研究中,我们从对照植物毒性试验的处理中收集了生长(以叶数和叶面积为基础的相对生长速率)和形态学(叶形和菌落平均大小)数据。所有分析的测试均以相同的方式进行螺旋藻(左)施莱德。(巨型浮萍)克隆(RDSC ID号5501)在相同的实验条件下进行了四年以上的测试。我们旨在评估上述参数的总体变异性,并测试内在的增长模式是否会在短期内影响增长数据。通常,该结果反映了长期所测参数的高度稳定性,但也表明某些时间上的变化是不可避免的,这可能会影响生长测试的可比性。基于叶面积的相对增长率导致变异系数小于通常首选的基于叶数的变异系数。结果还表明,平均增长率与其变异系数之间呈负相关。所以,建议在标准浮萍生长抑制试验中引入较高的最小生长速率和/或最大允许的对照培养物变异系数。按季节汇总的生长数据分析表明,与夏季和初秋相比,从中秋到春季中旬,实验室浮萍培养的生长更快且平均叶状体更大。但是,在短期内(约50天),我们没有观察到明显的生长趋势,这表明连续的叶代对这一时间范围内的生长特性没有影响。我们的研究结果指出了评估浮萍培养物中内在生长动态的重要性,也指出了已经收集的植物毒性数据在解决新的研究问题中的可重复使用性。按季节汇总的生长数据分析表明,与夏季和初秋相比,从中秋到春季中旬,实验室浮萍培养的生长更快且平均叶状体更大。但是,在短期内(约50天),我们没有观察到明显的生长趋势,这表明连续的叶代对这一时间范围内的生长特性没有影响。我们的研究结果指出了评估浮萍培养物中内在生长动态的重要性,也指出了已经收集的植物毒性数据在解决新的研究问题中的可重复使用性。按季节汇总的生长数据分析表明,与夏季和初秋相比,从中秋到春季中旬,实验室浮萍培养的生长更快且平均叶状体更大。但是,在短期内(约50天),我们没有观察到明显的生长趋势,这表明连续的叶代对这一时间范围内的生长特性没有影响。我们的结果指出,评估浮萍培养物中内在的生长动态的重要性,以及针对解决新的研究问题而已收集的植物毒性数据的可重复使用性。在较短的时间内(约50天),我们没有观察到明显的生长趋势,这表明连续的叶代对这一时间范围内的生长特性没有影响。我们的研究结果指出了评估浮萍培养物中内在生长动态的重要性,也指出了已经收集的植物毒性数据在解决新的研究问题中的可重复使用性。在较短的时间内(约50天),我们没有观察到明显的生长趋势,这表明连续的叶代对这一时间范围内的生长特性没有影响。我们的研究结果指出了评估浮萍培养物中内在生长动态的重要性,也指出了已经收集的植物毒性数据在解决新的研究问题中的可重复使用性。

更新日期:2018-05-28
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