Abstract This work presents the development of a sensitive and friendly environment electrochemical methodology with high analytical performance for quinolone detection. The method is based on square-wave anodic stripping voltammetry which is simple and reliable for determination of quinolone family on the bismuth film electrode (BiFE). The deposition potential, deposition time, buffer solution pH, voltammetry detection technique and bismuth concentration at fixed quinolone concentration (1 μg L−1) were evaluated by fractionated factorial and central composite designs. The high cross-reactivity obtained in the evaluation of different quinolones showed that this methodology could be applied to quinolone family with limits of detection (LOD) close to 0.5 ng L−1. Since is widely known that quinolones can reach aquatic ecosystems, the developed electroanalytical method was applied in the monitoring of moxifloxacin (MOXI) concentrations in ecotoxicity studies by means of acute toxicity test using two cladoceran species as biological models: Daphnia magna and Ceriodaphnia dubia. As a result of ecotoxicity, both species showed comparable sensitivities at MOXI. Toxicity studies of quinolones on aquatic organisms are very limited so the effects of MOXI to cladoceran species are highlighted. The interaction of both disciplines –Chemistry and Ecology- would allow knowing of global behavior of quinolones in natural environments, from an integrative perspective.

中文翻译：

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喹诺酮类检测电化学方法的发展：在枝角类动物生态毒性研究中的应用

摘要 这项工作提出了一种灵敏且友好的环境电化学方法的发展，该方法具有用于喹诺酮检测的高分析性能。该方法基于方波阳极溶出伏安法，简单可靠，用于铋膜电极 (BiFE) 上喹诺酮族的测定。沉积电位、沉积时间、缓冲溶液 pH 值、伏安法检测技术和固定喹诺酮浓度 (1 μg L-1) 下的铋浓度通过分级析因和中心复合设计进行评估。在评估不同喹诺酮类药物时获得的高交叉反应性表明，该方法可应用于检测限 (LOD) 接近 0.5 ng L-1 的喹诺酮类家族。众所周知，喹诺酮类药物可以进入水生生态系统，通过使用两种枝角类动物作为生物模型的急性毒性试验：Daphnia magna 和 Ceriodaphnia dubia，将开发的电分析方法应用于生态毒性研究中莫西沙星 (MOXI) 浓度的监测。由于生态毒性，这两个物种在 MOXI 中表现出相当的敏感性。喹诺酮类对水生生物的毒性研究非常有限，因此突出了 MOXI 对枝角类物种的影响。化学和生态学这两个学科的相互作用将允许从综合的角度了解喹诺酮类药物在自然环境中的全球行为。Daphnia magna 和 Ceriodaphnia dubia。由于生态毒性，这两个物种在 MOXI 中表现出相当的敏感性。喹诺酮类对水生生物的毒性研究非常有限，因此突出了 MOXI 对枝角类物种的影响。化学和生态学这两个学科的相互作用将允许从综合的角度了解喹诺酮类药物在自然环境中的全球行为。Daphnia magna 和 Ceriodaphnia dubia。由于生态毒性，这两个物种在 MOXI 中表现出相当的敏感性。喹诺酮类对水生生物的毒性研究非常有限，因此突出了 MOXI 对枝角类物种的影响。化学和生态学这两个学科的相互作用将允许从综合的角度了解喹诺酮类药物在自然环境中的全球行为。