Photogenerated Hole-Induced Chemical Redox Cycling on Bi2S3/Bi2Sn2O7 Heterojunction: Toward General Amplified Split-Type Photoelectrochemical Immunoassay.,ACS Sensors

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Photogenerated Hole-Induced Chemical Redox Cycling on Bi2S3/Bi2Sn2O7 Heterojunction: Toward General Amplified Split-Type Photoelectrochemical Immunoassay.
ACS Sensors ( IF 8.2 ) Pub Date : 2018-06-04 , DOI: 10.1021/acssensors.8b00332
Jun-Tao Cao ₁ , Bing Wang _{1,

2} , Yu-Xiang Dong ₁ , Qian Wang ₂ , Shu-Wei Ren ₃ , Yan-Ming Liu ₁ , Wei-Wei Zhao _{2,

4}

Affiliation

This work reports the elegant bridging of enzymatic generation of electron donor with photogenerated hole-induced chemical redox cycling amplification (RCA) for innovative photoelectrochemical (PEC) immunoassay, by the aid of a heterojunction photoelectrode with split-type strategy. Specifically, the system was exemplified by the alkaline phosphatase (ALP) catalytic generation of ascorbic acid (AA), the redox cycling of AA by tris (2-carboxyethyl) phosphine (TCEP) as reductant, and the use of a novel Bi2S3/Bi2Sn2O7 heterojunction and myoglobin (Myo) as the photoelectrode and the target, respectively. After the immunoreaction and ALP-induced production of AA, the subsequent oxidation of AA by the photogenerated holes of the Bi2S3/Bi2Sn2O7 heterojunction could be cycled via the regeneration of AA by TCEP from the oxidized product of dehydroascorbic acid, leading to easy signal amplification for the sensitive immunoassay of Myo in real samples. It is believed that this work provided a basis for further design and development of general RCA-based PEC immunoassays.

中文翻译：

Bi2S3 / Bi2Sn2O7异质结上的光生空穴诱导化学氧化还原循环：迈向一般放大的拆分型光电化学免疫分析。

这项工作报告了借助异质结光电极和分体式策略，利用光生空穴诱导的化学氧化还原循环扩增（RCA）进行电子供体酶促生成的优雅桥接，用于创新的光电化学（PEC）免疫测定。具体而言，该系统的示例为抗坏血酸（AA）的碱性磷酸酶（ALP）催化生成，三（2-羧乙基）膦（TCEP）作为还原剂对AA的氧化还原循环以及新型Bi2S3 / Bi2Sn2O7的使用异质结和肌红蛋白（Myo）分别作为光电极和目标。免疫反应和ALP诱导的AA产生后，Bi2S3 / Bi2Sn2O7异质结的光生空穴随后对AA的氧化可以通过TCEP从脱氢抗坏血酸的氧化产物再生AA来循环进行，从而可以轻松地放大信号，用于真实样品中Myo的灵敏免疫测定。相信这项工作为进一步设计和开发基于RCA的常规PEC免疫测定法提供了基础。

更新日期：2018-05-31

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