Combined scanning electrochemical and fluorescence microscopies using a tetrazine as a single redox and luminescent (electrofluorochromic) probe†,Chemical Science

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Combined scanning electrochemical and fluorescence microscopies using a tetrazine as a single redox and luminescent (electrofluorochromic) probe†
Chemical Science ( IF 7.6 ) Pub Date : 2018-05-30 00:00:00 , DOI: 10.1039/c8sc01814f
L Guerret-Legras ₁ , J F Audibert ₁ , G V Dubacheva ₁ , F Miomandre ₁

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The possibility of using a single electroactive and luminescent molecule both as a redox mediator and as a fluorophore in an experiment combining in situ Scanning Electrochemical Microscopy (SECM) and epifluorescence microscopy was validated. The usual working modes of SECM, namely positive and negative feedback as well as generation–collection, were used and the fluorescence images, intensity and spectra were recorded for each configuration. The tip potential, tip–substrate distance and, in the case of a conducting substrate, the substrate potential are the parameters that are likely to control the fluorescence. It is shown that the tip can be used to switch on and off the luminescence and that the modulation amplitude maximum is sensitive to the nature of the substrate. Approach curves based on this fluorescence modulation amplitude can be obtained showing a higher sensitivity than the classical electrochemical ones.

中文翻译：

使用四嗪作为单一氧化还原和发光（电荧光变色）探针的组合扫描电化学和荧光显微镜†

在结合原位扫描电化学显微镜 (SECM) 和落射荧光显微镜的实验中，验证了使用单一电活性和发光分子作为氧化还原介体和荧光团的可能性。使用SECM的常用工作模式，即正反馈和负反馈以及生成-收集，并记录每种配置的荧光图像、强度和光谱。尖端电位、尖端-基底距离以及导电基底的情况下的基底电位是可能控制荧光的参数。结果表明，尖端可用于打开和关闭发光，并且调制幅度最大值对基底的性质敏感。可以获得基于这种荧光调制幅度的接近曲线，显示出比经典电化学曲线更高的灵敏度。

更新日期：2018-05-30

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