Nanomechanics of Pectin-Linked β-Lactoglobulin Nanofibril Bundles,Biomacromolecules

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Nanomechanics of Pectin-Linked β-Lactoglobulin Nanofibril Bundles
Biomacromolecules ( IF 5.5 ) Pub Date : 2018-05-29 00:00:00 , DOI: 10.1021/acs.biomac.8b00408
Simon M. Loveday _{1,

2} , A. Patrick Gunning ₃

Affiliation

Nanofibrils of β-lactoglobulin can be assembled into bundles by site-specific noncovalent cross-linking with high-methoxyl pectin (Hettiarachchi et al. Soft Matter2016, 12, 756). Here we characterized the nanomechanical properties of bundles using atomic force microscopy and force spectroscopy. Bundles had Gaussian cross sections and a mean height of 17.4 ± 1.4 nm. Persistence lengths were calculated using image analysis with the mean-squared end-to-end model. The relationship between the persistence length and the thickness had exponents of 1.69–2.30, which is consistent with previous reports for other fibril types. In force spectroscopy experiments, the bundles stretched in a qualitatively different manner to fibrils, and some of the force curves were consistent with peeling fibrils away from bundles. The flexibility of pectin-linked nanofibril bundles is likely to be tunable by modulating the stiffness and length of fibrils and the ratio of pectin to fibrils, giving rise to a wide range of structures and functionalities.

中文翻译：

果胶连接的β-乳球蛋白纳米原纤维束的纳米力学。

β乳球蛋白的纳米纤丝可以通过被组装成束位点特异性的非共价交联的高甲氧基果胶（Hettiarachchi等软物质2016，12（756）。在这里，我们使用原子力显微镜和力谱表征了束的纳米力学性能。束具有高斯截面，平均高度为17.4±1.4 nm。持久性长度是使用均方根端到端模型通过图像分析计算得出的。持续长度与厚度之间的关系的指数为1.69–2.30，这与先前针对其他原纤维类型的报道一致。在力谱实验中，束以质性不同的方式拉伸成原纤维，并且某些力曲线与将原纤维从束上剥离下来是一致的。通过调节原纤维的刚度和长度以及果胶与原纤维的比例，可以调节果胶连接的纳米原纤维束的柔韧性，

更新日期：2018-05-29

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