Abstract Green synthesis of the Platinum nanoparticle of dates is carried out for examining their effect on various cancer cells. The extract solution of Dates (biodegradable surfactant) is used for this purpose. The bio-degradable plant-based surfactant, used in the study, occurs naturally, and no other reducing, or capping agent is used for cancer cell treatment. The aqueous extract solution of popular dates Ajwa and Barni acts as a stabilizing and reducing agent during the production of PtNPs at ambient condition because of simplicity, long-time stability, and cost-effectiveness. In order to achieve the best size and shape of nanoparticles, different ratio of extract and metal salt were mixed and developed. Additionally, nanoparticles of varying size were furnished by altering the pH of the reaction. Spectroscopic techniques like FTIR, X-ray Diffraction (XRD), EDX, thermos-gravimetric analysis (TGA), UV–vis, and transmission electron microscopy (TEM) were applied to identify PtNPs. In this study, electrochemical HPCL and high-performance liquid chromatography (HPCL) are combined for better understanding and effectiveness. The metabolites such as amino acid, sugar, organic acid, flavonoids, phenol, and minerals, in the Dates produced in Al-Madinah Al-Munawarah, have been analyzed with the help of the techniques employed in the study. PtNPs' anticancer activities were evaluated for different cancer cells including the colon carcinoma cells (HCT-116), breast cells (MCF-7), and hepatocellular carcinoma (HePG-2). Commonly used effective anticancer agent, Doxorubicin HCl, is used in the current study related to anticancer activitiy. To discover the antibacterial effect, antibacterial agents Ampicillin and Gentamicin are used. Lastly, the Gram-negative bacteria: Escherichia coli (RCMB 010052) and Gram-Positive Bacteria: Bacillus subtilis (RCMB 010067) were used to determine the antibacterial application of PtNPs.

中文翻译：

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使用沙特枣提取物绿色合成铂纳米颗粒及其在癌细胞治疗中的应用

摘要 进行了椰枣铂纳米颗粒的绿色合成，以研究它们对各种癌细胞的影响。为此使用枣子（可生物降解的表面活性剂）的提取液。研究中使用的可生物降解的植物基表面活性剂是天然存在的，没有其他还原剂或封端剂用于癌细胞治疗。由于简单、长期稳定性和成本效益，流行枣 Ajwa 和 Barni 的水提取液在环境条件下生产 PtNPs 期间充当稳定剂和还原剂。为了获得最佳尺寸和形状的纳米颗粒，混合开发了不同比例的提取物和金属盐。此外，通过改变反应的 pH 值来提供不同尺寸的纳米颗粒。光谱技术，如 FTIR，应用 X 射线衍射 (XRD)、EDX、热重分析 (TGA)、紫外-可见光和透射电子显微镜 (TEM) 来鉴定 PtNP。在本研究中，电化学 HPCL 和高效液相色谱 (HPCL) 相结合，以更好地理解和提高有效性。在研究中使用的技术的帮助下，分析了 Al-Madinah Al-Munawarah 生产的枣中的氨基酸、糖、有机酸、黄酮类化合物、苯酚和矿物质等代谢物。评估了 PtNPs 对不同癌细胞的抗癌活性，包括结肠癌细胞 (HCT-116)、乳腺细胞 (MCF-7) 和肝细胞癌 (HePG-2)。常用的有效抗癌剂盐酸多柔比星用于当前与抗癌活性相关的研究。为了发现抗菌作用，使用抗菌剂氨苄青霉素和庆大霉素。最后，使用革兰氏阴性菌：大肠杆菌（RCMB 010052）和革兰氏阳性菌：枯草芽孢杆菌（RCMB 010067）来确定 PtNPs 的抗菌应用。