A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood†,Lab on a Chip

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A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood†
Lab on a Chip ( IF 6.1 ) Pub Date : 2018-05-29 00:00:00 , DOI: 10.1039/c8lc00371h
Wenhan Liu _{1,

2,

3,

4} , Jagotamoy Das _{2,

3,

5,

6,

7} , Adam H. Mepham _{1,

2,

3,

4} , Carine R. Nemr _{2,

3,

8,

9,

10} , Edward H. Sargent _{2,

3,

11,

12,

13} , Shana O. Kelley _{1,

2,

3,

4,

5}

Affiliation

Integrated devices for automated nucleic acid testing (NAT) are critical for infectious disease diagnosis to be performed outside of centralized laboratories. The gold standard methods for NAT are enzymatic amplification methods like the polymerase chain reaction that typically require expensive equipment and highly-trained personnel, limiting use in low-resource settings. A low-cost, integrated, rapid, portable and user-friendly point-of-care (POC) nucleic acid diagnostic device will improve the accessibility of NAT. Here, we present a fully integrated and simple-to-use POC device operated by a passive fluidic method that is able to perform a sequential multi-step assay to detect viral nucleic acids in blood. This simple device enabled the rapid detection of hepatitis C virus in blood in approximately 30 minutes with minimal sample handling by the user.

中文翻译：

完全集成的自动化测试设备，可在全血中检测无PCR的病毒核酸†

自动核酸检测（NAT）的集成设备对于在集中实验室之外进行传染病诊断至关重要。NAT的金标准方法是酶促扩增方法，例如聚合酶链反应，通常需要昂贵的设备和训练有素的人员，从而限制了在资源贫乏地区的使用。低成本，集成，快速，便携式且用户友好的即时护理（POC）核酸诊断设备将改善NAT的可访问性。在这里，我们介绍了一种通过被动流体方法操作的完全集成且易于使用的POC设备，该设备能够执行顺序的多步分析以检测血液中的病毒核酸。

更新日期：2018-05-29

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