Vinylboronic acids (VBAs) are stable, unstrained bioorthogonal reactants in tetrazine ligation. Fast reaction rates are obtained for tetrazines containing a coordinating substituent, whereas VBAs lacking a Lewis basic substituent are unreactive. We explored the molecular basis of the unique reactivity of VBAs and demonstrate the scope in the simultaneous orthogonal tetrazine labelling of two proteins.

中文翻译：

---

配位辅助的生物正交化学：用乙烯基硼酸和应变烯烃对正交四嗪进行连接

乙烯基硼酸（VBA）在四嗪连接中是稳定的，未拉伸的生物正交反应物。对于含有配位取代基的四嗪，可获得快速的反应速率，而缺少路易斯碱性取代基的VBA则不具有反应性。我们探索了VBAs独特反应性的分子基础，并证明了两种蛋白质同时正交四嗪标记的范围。