A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria,Angewandte Chemie International Edition

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A Multifunctional Chemical Probe for the Measurement of Local Micropolarity and Microviscosity in Mitochondria
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-06-19 , DOI: 10.1002/anie.201802796
Arturo Jiménez-Sánchez ₁ , Eric K. Lei ₁ , Shana O. Kelley _{1,

2}

Affiliation

The measurement of physicochemical parameters in living cells can provide information on individual cellular organelles, helping us to understand subcellular function in health and disease. While organelle‐specific chemical probes have allowed qualitative evaluation of microenvironmental variations, the simultaneous quantification of mitochondrial local microviscosity (η_m) and micropolarity (ϵ_m), along with concurrent structural variations, has remained an unmet need. Herein, we describe a new multifunctional mitochondrial probe (MMP) for simultaneous monitoring of η_m and ϵ_m by fluorescence lifetime and emission intensity recordings, respectively. The MMP enables highly precise measurements of η_m and ϵ_m in the presence of a variety of agents perturbing cellular function, and the observed changes can also be correlated with alterations in mitochondrial network morphology and motility. This strategy represents a promising tool for the analysis of subtle changes in organellar structure.

中文翻译：

用于检测线粒体局部微极性和微粘度的多功能化学探针

活细胞中理化参数的测量可以提供有关单个细胞器的信息，帮助我们了解健康和疾病中的亚细胞功能。虽然细胞器特异性化学探针已经允许微环境变化的定性评价，线粒体本地微粘度（的同时定量η_米）和微极性（ε_米），并发结构变化一起，仍然是一个未满足的需要。在此，我们描述了一种新的多功能线粒体探针（MMP），同时进行监视的η_米和ε_米分别由荧光寿命和发射强度的记录。这MMP使得能够高度精确的测量η_米和ε_米中的各种试剂扰乱细胞功能的存在，并且所观察到的变化也可以与线粒体网络形态和运动改变相关。该策略代表了分析细胞器结构细微变化的有前途的工具。

更新日期：2018-06-19

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