We review the use of luminescent nanoparticles in super-resolution imaging and single-molecule tracking, and showcase novel approaches to super-resolution imaging that leverage the brightness, stability, and unique optical-switching properties of these nanoparticles. We also discuss the challenges associated with their use in biological systems, including intracellular delivery and molecular targeting. In doing so, we hope to provide practical guidance for biologists and continue to bridge the fields of super-resolution imaging and nanoparticle engineering to support their mutual advancement.

中文翻译：

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用于超分辨率显微镜和单分子跟踪的纳米颗粒

我们回顾了发光纳米粒子在超分辨率成像和单分子跟踪中的使用，并展示了利用这些纳米粒子的亮度，稳定性和独特的光学开关特性的超分辨率成像新方法。我们还将讨论与它们在生物系统中使用相关的挑战，包括细胞内递送和分子靶向。在此过程中，我们希望为生物学家提供实用的指导，并继续在超分辨率成像和纳米粒子工程领域架起桥梁，以支持他们的共同进步。