Abstract A highly selective, sensitive yet simple colorimetric assay was developed for Se(IV) detection based on the silver nanoparticles (AgNPs), with hydride generation for separation and sample introduction. The proposed method could be easily performed by introducing the hydride vapor of the analyte (H2Se) into a thioglycolic acid (TGA) capped AgNPs solution. A combination of experimental and theoretical studies was used to illustrate the mechanism of this AgNPs colorimetric assay. The strong interaction of AgNPs with Se0 (oxidation product of H2Se) and/or H2Se weakens the stabilizing effect of TGA on AgNPs, leading to the aggregation of AgNPs and thus the color change of the AgNPs solution from bright yellow to light yellow or even colorless with the increase of Se(IV) concentration. The limit of detection was found to be 5 μg L−1 with UV–vis spectrophotometric detection, and 50 μg L−1 with the naked eye visual observation. The proposed method showed excellent selectivity and was successfully applied for Se(IV) determination in fish protein and sea water samples.

中文翻译：

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基于银纳米颗粒的痕量硒比色分析，用于样品引入的氢化物生成

摘要 基于银纳米粒子 (AgNPs)，开发了一种高选择性、灵敏且简单的比色检测方法，用于 Se(IV) 检测，生成氢化物以进行分离和样品引入。通过将分析物 (H2Se) 的氢化物蒸气引入巯基乙酸 (TGA) 封端的 AgNPs 溶液中，可以轻松地执行所提出的方法。实验和理论研究的结合被用来说明这种 AgNPs 比色测定的机制。AgNPs与SeO（H2Se的氧化产物）和/或H2Se的强相互作用减弱了TGA对AgNPs的稳定作用，导致AgNPs聚集，从而使AgNPs溶液的颜色从亮黄色变为淡黄色甚至无色随着 Se(IV) 浓度的增加。紫外-可见分光光度法检测的检测限为 5 μg L-1，肉眼观察的检测限为 50 μg L-1。所提出的方法显示出优异的选择性，并成功应用于鱼类蛋白质和海水样品中 Se(IV) 的测定。