Maize, a crop cultivated worldwide, was investigated for plant tissue and crop stage specific colonization of endophytic bacteria. Such bacterial interactions have high potential to enhance maize grain yield by means of biological nitrogen fixation and/or plant growth promoting activities. In this study endophytic bacteria were isolated from a hybrid PEEHM-5 and composite PC-4 maize varieties using root, stem and leaf tissues of plants at vegetative, flowering and maturity stages of growth. PEEHM-5 harbored higher endophytic bacterial population than PC-4 at all growth stages, with highest in roots and at flowering stage. Morphologically 188 different endophytic isolates (82 from PEEHM-5, 106 from PC-4) were screened for plant growth promoting attributes viz. P, K, Zn solubilization, production of hormones, siderophore, ACC deaminase, HCN, biological nitrogen fixation and biocontrol of two maize fungal pathogens. Thirty one potential PGP isolates on RFLP analysis of their amplified 16S rRNA gene, were clustered in 13 phylogenetic groups. On sequencing and blasting of amplified 16S rRNA gene of representative isolates from each group identified PC-4 endophytic bacterial isolates as Bacillus aryabhattai, Pantoea cypripedii, Bacillus licheniformis, Klebsiella sp., Pantoea dispersa, Klebsiella variicola, Pantoea sp., Agrobacterium larrymoorei and PEEHM-5 endophytic bacterial isolates as Bacillus sp., Bacillus amyloliquefaciens, Lactococcus lactis, Bacillus cereus and Staphylococcus hominis. In planta evaluation of potential isolates at variable chemical fertilizer input indicated their potential in compensating nearly 25% of the fertilizer input as observed on their improvement of shoot and root parameters. Lactococcus lactis inoculation influenced maximum followed by Pantoea and Klebsiella isolates.

玉米是一种在全球范围内种植的作物，其植物组织和作物阶段内生细菌的特定定殖研究得到了研究。这种细菌相互作用具有通过生物固氮和/或促进植物生长的活性来提高玉米籽粒产量的高潜力。在这项研究中，使用处于营养，开花和成熟阶段的植物的根，茎和叶组织从杂种PEEHM-5和PC-4复合玉米品种中分离出内生细菌。在所有生长期，PEEEM-5的内生细菌数量均高于PC-4，其根和花期最高。从形态学上筛选了188种不同的内生分离株（PEEEM-5中的82种，PC-4中的106种）以寻找促进植物生长的特性。P，K，Zn增溶，激素产生，铁载体，ACC脱氨酶，HCN，生物固氮和两种玉米真菌病原体的生物防治。根据对扩增后的16S rRNA基因的RFLP分析，将31个潜在的PGP分离物聚集在13个系统发育组中。测序并从识别的PC-4内生细菌分离以各组代表菌株扩增16S rRNA基因的爆破aryabhattai芽孢杆菌，泛菌属cypripedii，地衣芽孢杆菌，克雷伯氏菌属，分散泛菌，克雷伯氏菌属variicola，泛菌属SP 。，农杆菌larrymoorei和PEEHM -5内生细菌分离株，如芽孢杆菌属，解淀粉芽孢杆菌，乳酸乳球菌，蜡状芽孢杆菌和人葡萄球菌。在植物中，对可变化学肥料输入量下潜在分离株的评估表明，如其枝条和根部参数的改善所观察到的，它们有潜力补偿近25％的肥料输入量。乳酸乳球菌的接种影响最大，其次是泛菌和克雷伯菌。