Abstract Published data from different techniques used in the analysis of the main biomolecular families (lignin, carbohydrates and lipids) in 100 cm depth peat cores from the Sagnes peat bog (Limousin, France) were compared. Thermochemolysis using tetramethylammonium hydroxide was compared with the classical chemical degradation methods for lignin (CuO-NaOH oxidation) and carbohydrates (acid hydrolysis with HCl), and the Bligh and Dyer extraction method for lipids. Differences observed between the methods are mostly due to the different degradation mechanisms involved in each method. For lignin, the selective cleavage of β O 4 aryl ether bonds during thermochemolysis allowed identification of a more oxidized/degraded lignin pool in the deepest part of the peat core. For carbohydrates, the capacity of thermochemolysis to detect free (or terminal) carbohydrates showed an increase in microbial contribution at the interfaces between three distinct peat layers: the acrotelm, the upper part of the peat permanently above the water table; the mesotelm, the layer where water table fluctuations occur; and the catotelm, the bottom layer of peat that is permanently below the water table. For the lipid fraction, thermochemolysis revealed a lipid pool that was more influenced by microbial reworking. These results imply that, depending on the techniques used, the interpretation of the extent of degradation or source could differ. Therefore, TMAH thermochemolysis could be viewed and used as a complementary method of analysis, offering additional molecular data and contributing to a better understanding of the nature and sedimentation processes of organic matter in peat bogs.

中文翻译：

---

用于分析泥炭沼泽中碳水化合物、木质素和脂质的热化学分解和经典化学降解和提取方法的比较

摘要 对来自 Sagnes 泥炭沼泽（法国利穆赞）的 100 cm 深泥炭核心中主要生物分子家族（木质素、碳水化合物和脂质）的分析所用不同技术的已发表数据进行了比较。将使用四甲基氢氧化铵的热化学分解与木质素（CuO-NaOH 氧化）和碳水化合物（用 HCl 酸水解）的经典化学降解方法以及脂质的 Bligh 和 Dyer 提取方法进行了比较。观察到的方法之间的差异主要是由于每种方法所涉及的降解机制不同。对于木质素，热化学分解过程中 β O 4 芳基醚键的选择性裂解允许在泥炭核心的最深处识别出更多氧化/降解的木质素库。对于碳水化合物，热化学分解检测游离（或末端）碳水化合物的能力表明，微生物在三个不同泥炭层之间的界面处增加了： acrotelm，泥炭的上部，永久高于地下水位；中层，发生地下水位波动的层；和 catotelm，泥炭的底层，永久低于地下水位。对于脂质部分，热化学分解揭示了一个受微生物再加工影响更大的脂质池。这些结果意味着，根据所使用的技术，对降解程度或来源的解释可能会有所不同。因此，可以将 TMAH 热化学分解视为一种补充分析方法，