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Deep-Red AIE-Active Fluorophore for Hypochlorite Detection and Bioimaging in Live Cells
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-06-01 , DOI: 10.1021/acs.iecr.8b01071 Lei Wang 1 , Xiuli Chen 1 , Qi Xia , Ruiyuan Liu , Jinqing Qu 1
Industrial & Engineering Chemistry Research ( IF 3.8 ) Pub Date : 2018-06-01 , DOI: 10.1021/acs.iecr.8b01071 Lei Wang 1 , Xiuli Chen 1 , Qi Xia , Ruiyuan Liu , Jinqing Qu 1
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Hypochlorite (ClO–) is widely presented in daily life and human body. An appropriate amount of hypochlorite may help the body regulate the immune system, while excessive hypochlorite can cause a variety of diseases. Herein, a novel fluorescence probe PTZ-BT containing a large conjugate plane was designed and synthesized. It exhibited a large Stokes shift of 158 nm and extreme AIE activity and emitted deep-red fluorescence (∼642 nm). Moreover, PTZ-BT showed specific turn-off fluorescent-responsive and high-sensitive detection (detection limit equals to 0.64 μM) toward ClO– in PBS buffer solutions. Job’s plot showed that the binding stoichiometry of PTZ-BT with ClO– was 1:1. It was further demonstrated that sulfur atoms in the phenothiazine groups were oxidized to sulfoxide by ClO–, resulting in fluorescence quenching supported by high-resolution mass spectrometry. In addition, PTZ-BT was used for bioimaging in HepG-2 cells with low cell toxicity and good cell permeability. Furthermore, live cells studies also demonstrated PTZ-BT can detect exogenous ClO– inside live cells.
中文翻译：
深红色AIE活性荧光团用于活细胞中的次氯酸盐检测和生物成像
次氯酸盐（ClO –）在日常生活和人体中广泛存在。适量的次氯酸盐可帮助机体调节免疫系统，而过量的次氯酸盐可引起多种疾病。本文中，设计并合成了一种新的包含大共轭平面的荧光探针PTZ-BT。它表现出158 nm的大斯托克斯位移和极高的AIE活性，并发出深红色荧光（〜642 nm）。此外，PTZ-BT显示特定关断荧光响应和高灵敏度的检测（检测极限等于0.64μM）朝向CLO -在PBS缓冲液溶液。乔布的图表明，PTZ-BT与ClO的结合化学计量–是1：1。进一步证明，在吩噻嗪基硫原子通过CLO氧化为亚砜- ，导致由高分辨率质谱支持荧光淬灭。此外，PTZ-BT用于HepG-2细胞的生物成像，具有低细胞毒性和良好的细胞通透性。此外，活细胞的研究也证明了PTZ-BT可以检测外源性CLO -里面活细胞。
更新日期：2018-06-01
中文翻译：
深红色AIE活性荧光团用于活细胞中的次氯酸盐检测和生物成像
次氯酸盐（ClO –）在日常生活和人体中广泛存在。适量的次氯酸盐可帮助机体调节免疫系统，而过量的次氯酸盐可引起多种疾病。本文中，设计并合成了一种新的包含大共轭平面的荧光探针PTZ-BT。它表现出158 nm的大斯托克斯位移和极高的AIE活性，并发出深红色荧光（〜642 nm）。此外，PTZ-BT显示特定关断荧光响应和高灵敏度的检测（检测极限等于0.64μM）朝向CLO -在PBS缓冲液溶液。乔布的图表明，PTZ-BT与ClO的结合化学计量–是1：1。进一步证明，在吩噻嗪基硫原子通过CLO氧化为亚砜- ，导致由高分辨率质谱支持荧光淬灭。此外，PTZ-BT用于HepG-2细胞的生物成像，具有低细胞毒性和良好的细胞通透性。此外，活细胞的研究也证明了PTZ-BT可以检测外源性CLO -里面活细胞。
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