A thorough assessment of the microstructural changes and synergistic effects of hydrothermal and/or ultrafine grinding pretreatment on the subsequent enzymatic hydrolysis of corn stover was performed in this study. The mechanism of pretreatment was elucidated by characterizing the particle size, specific surface area (SSA), pore volume (PV), average pore size, cellulose crystallinity (CrI) and surface morphology of the pretreated samples. In addition, the underlying relationships between the structural parameters and final glucose yields were elucidated, and the relative significance of the factors influencing enzymatic hydrolyzability were assessed by principal component analysis (PCA). Hydrothermal pretreatment at a lower temperature (170 °C) combined with ultrafine grinding achieved a high glucose yield (80.36%) at a low enzyme loading (5 filter paper unit (FPU)/g substrate) which is favorable. The relative significance of structural parameters in enzymatic hydrolyzability was SSA > PV > average pore size > CrI/cellulose > particle size. PV and SSA exhibited logarithmic correlations with the final enzymatic hydrolysis yield.

在这项研究中，对水热和/或超细粉碎预处理对随后的玉米秸秆酶水解的微观结构变化和协同作用进行了全面的评估。通过表征预处理样品的粒度，比表面积（SSA），孔体积（PV），平均孔径，纤维素结晶度（CrI）和表面形态，阐明了预处理的机理。此外，阐明了结构参数与最终葡萄糖产量之间的潜在关系，并通过主成分分析（PCA）评估了影响酶水解性的因素的相对重要性。较低温度（170°C）的水热预处理与超细粉碎相结合可实现较高的葡萄糖收率（80。低酶含量（5滤纸单位（FPU）/ g底物）时是有利的（36％）。结构参数在酶水解性中的相对重要性为：SSA> PV>平均孔径> CrI /纤维素>粒度。PV和SSA表现出与最终酶水解产率的对数关系。