Probiotic bacteria have the ability to alter immune response conferring treatment to inflammatory diseases. In the current study, probiotic Lactobacillus strains were subjected to adhesion to epithelial Caco-2 cells. Subsequently, gene expression of pro-and anti-inflammatory genes were studied under three stimulation conditions (which includes LPS-challenge, stress induction and pathogens invasion). Herein, immunomodulatory activity of native Lactobacillus strains (n = 3) were compared to L. rhamnosus GG. The studied strains had down-regulated the gene expression of pro-inflammatory cytokines (CKs) (like TNF-α, IL-1α, IL-1β, IL-6 and IL-8) and up-regulated the anti-inflammatory CKs like TGFβ1, IL-4, and IL-10. Gene expression of TLR2 has also been noted. Strain-specific interactions were noticed during the different probiotic treatment. Further, phylogenetic dendrogram of mucin adhesion domain suggested the interaction of Lactobacillus and Listeria species, explaining competitive colonization and exclusion mechanism in an ecological niche. Overall, isolate L. paraplantarum MTCC 9483 has effectively modulated induced conditions by its anti-inflammatory activity.

益生菌具有改变免疫应答的能力，从而赋予炎性疾病治疗。在当前的研究中，益生菌乳酸菌菌株粘附于上皮Caco-2细胞。随后，在三种刺激条件下（包括LPS挑战，应激诱导和病原体入侵）研究了促炎基因和抗炎基因的基因表达。在此，将天然乳酸杆菌菌株（n = 3）的免疫调节活性与鼠李糖乳杆菌进行了比较。GG。所研究的菌株下调了促炎细胞因子（CKs）（如TNF-α，IL-1α，IL-1β，IL-6和IL-8）的基因表达，并上调了抗炎性CKs（如TNF-α，IL-1α，IL-1β，IL-6和IL-8）。 TGFβ1，IL-4和IL-10。还已经注意到TLR2的基因表达。在不同的益生菌治疗过程中注意到菌株特异性相互作用。此外，粘蛋白粘附域的系统发育树状图表明乳杆菌和李斯特菌物种之间的相互作用，解释了生态位中的竞争性定居和排斥机制。总体而言，分离株副植物乳杆菌MTCC 9483通过其抗炎活性已有效地调节了诱发条件。