The mechanisms underlying beneficial actions of Rutin, a food derived-polyphenolic bioflavonoid, against myocardial ischemia-reperfusion (MI/R) injury were detected. We found that Rutin could significantly ameliorate infarct size and attenuate cardiac dysfunctionto in some extent. Additionally, Rutin could also improve variation of myocardial zymogram and up-regulate antioxidant systems in heart. The selective SIRT1 inhibitor, Ex-527, exacerbated MI/R injury as evidenced by further increased oxidative stress and infracted area in heart tissue. Importantly, Ex-527 abolished the cardioprotective effect of Rutin in MI/R rats. Docking analysis further demonstrated the vital role of SIRT1 on Rutin's cardioprotective effect. We found that activation of SIRT1/Nrf2 signaling induced by Rutin contributes to the reduced oxidative stress, along with decreased apoptosis of cardiomyocytes in heart tissue. Overall, our findings suggest that SIRT1/Nrf2 signaling pathway may be a potential therapeutic target for the treatment of oxidative stress and apoptosis related myocardial diseases.

发现了芦丁（一种食品衍生的多酚类生物类黄酮）对心肌缺血-再灌注（MI / R）损伤的有益作用的潜在机制。我们发现芦丁可以在一定程度上显着改善梗塞面积并减轻心脏功能障碍。此外，芦丁还可以改善心肌酶谱的变化并上调心脏中的抗氧化系统。选择性SIRT1抑制剂Ex-527加剧了MI / R损伤，这一点可通过进一步增加心脏组织中的氧化应激和侵入面积来证明。重要的是，Ex-527取消了芦丁对MI / R大鼠的心脏保护作用。对接分析进一步证明了SIRT1在芦丁的心脏保护作用中的重要作用。我们发现，芦丁诱导SIRT1 / Nrf2信号的激活有助于降低氧化应激，以及心脏组织中心肌细胞凋亡的减少。总体而言，我们的发现表明SIRT1 / Nrf2信号通路可能是治疗氧化应激和凋亡相关心肌疾病的潜在治疗靶标。