A new water-soluble polysaccharide (FVP1) was extracted from Flammulina velutipes by traditional method “water extraction and alcohol precipitation” and purified by column chromatography. Physicochemical characterization showed that FVP1 was a homogeneous polysaccharide with a relative molecular weight of 54.78 kDa. It is composed of mannose (7.74%), glucose (70.41%), and galactose (16.38%). FVP1 (1000 mg/ml) possessed significant immune activity by increasing the secretion of nitric oxide (NO), tumor necrosis factor-α (TNF-α) (3183 ± 133.84 pg/mL), interleukin (IL)-6 (1133.21 ± 39.05 pg/mL), and IL-12 (579.96 ± 74.53 pg/mL) in macrophages. Furthermore, FVP1 showed significant hepatitis B surface antibody (anti-HBV) activity through reducing the expression of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA replication. These results suggest a novel role for FVP1 to be applied as an immunomodulators in dietary supplements to prevent HBV infection.

从金针菇中提取一种新的水溶性多糖（FVP1）通过传统方法“水萃取和乙醇沉淀”，并通过柱色谱法纯化。理化性质表明，FVP1为均一多糖，相对分子量为54.78kDa。它由甘露糖（7.74％），葡萄糖（70.41％）和半乳糖（16.38％）组成。FVP1（1000 mg / ml）通过增加一氧化氮（NO），肿瘤坏死因子-α（TNF-α）（3183±133.84 pg / mL），白介素（IL）-6（1133.21± 39.05 pg / mL）和巨噬细胞中的IL-12（579.96±74.53 pg / mL）。此外，FVP1通过降低乙型肝炎表面抗原（HBsAg），乙型肝炎e抗原（HBeAg）和乙型肝炎病毒（HBV）DNA复制的表达，显示出显着的乙型肝炎表面抗体（anti-HBV）活性。