Abstract The present study aimed to develop an enzymatic colorimetric method using a surface-adsorbing biosensor to detect and kill bacteria in a single, simple, and rapid assay. The phosphorylated fluorescent probe 2-hydroxychalcone (HCAP) conjugated with an adhesive cationic polymer was designed (HCAP-PVP), which yielded greenish-yellow emission in aqueous buffer. Upon introduction of Escherichia coli and Staphylococcus aureus, the phosphate group inside the HCAP was cleaved by endogenous alkaline phosphatase (ALP) and the greenish-yellow emission ratiometrically changed to deep-red emission. This biosensor system detected bacteria over a wide range of bacterial densities (101–107 colony-forming units/mL) after 60 min, with similar bacterial detection abilities between aqueous- and solid-phase assays. Furthermore, the presence of a quaternary ammonium of dodecane in this system displayed efficient antibacterial activity because of the change in cellular hydrophobic interactions, which enabled this material to act as a dual sensor and killing material. Thus, this system is a novel, rapid, and simple enzymatic sensor with high sensitivity that can be used as a solid-based platform to detect and directly eliminate bacteria.

中文翻译：

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用于比色细菌检测的碱性磷酸酶响应荧光聚合物探针涂层表面

摘要 本研究旨在开发一种酶促比色法，使用表面吸附生物传感器在单一、简单和快速的测定中检测和杀死细菌。设计了与粘性阳离子聚合物结合的磷酸化荧光探针 2-羟基查尔酮 (HCAP) (HCAP-PVP)，其在水性缓冲液中产生黄绿色发光。引入大肠杆菌和金黄色葡萄球菌后，HCAP 内的磷酸基团被内源性碱性磷酸酶 (ALP) 裂解，黄绿色发射成比例地变为深红色发射。该生物传感器系统在 60 分钟后检测到细菌密度范围很广（101-107 菌落形成单位/mL），在水相和固相检测之间具有相似的细菌检测能力。此外，由于细胞疏水相互作用的变化，该系统中十二烷季铵的存在显示出有效的抗菌活性，这使该材料能够充当双重传感器和杀灭材料。因此，该系统是一种新型、快速、简单的酶传感器，具有高灵敏度，可用作检测和直接消除细菌的固体平台。