An interferometric nanobiosensor for the specific and label-free detection of the pollutant Irgarol 1051 directly in seawater has been settled. Due to the low molecular weight of Irgarol pollutant and its expected low concentration in seawater, the sensor is based on a competitive inhibition immunoassay. Parameters as surface biofunctionalization, concentration of the selective antibody and regeneration conditions have been carefully evaluated. The optimized immunosensor shows a limit of detection of only 3 ng/L, well below the 16 ng/L set by the EU as the maximum allowable concentration in seawater. It can properly operate during 30 assay-regeneration cycles using the same sensor biosurface and with a time-to-result of only 20 min for each cycle. Moreover, the interferometric nanosensor is able to directly detect low concentrations of Irgarol 1051 in seawater without requiring sample pre-treatments and without showing any background signal due to sea matrix effect.

已经建立了一种干涉纳米生物传感器，用于直接，无特异性地直接检测海水中的污染物Irgarol 1051。由于Irgarol污染物的分子量低且其在海水中的预期浓度低，因此该传感器基于竞争性抑制免疫测定。已经仔细评估了诸如表面生物功能化，选择性抗体的浓度和再生条件的参数。经过优化的免疫传感器的检测极限仅为3 ng / L，远低于EU设定为海水中最大允许浓度的16 ng / L。它可以使用相同的传感器生物表面在30个测定再生循环中正常运行，每个循环的结果时间仅为20分钟。而且，