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Characterization and Quantification of Oligosaccharides in Human Milk and Infant Formula
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-05-25 00:00:00 , DOI: 10.1021/acs.jafc.8b01515 Rose M. Nijman 1 , Yan Liu 2 , Apichaya Bunyatratchata 2 , Jennifer T. Smilowitz 2 , Bernd Stahl 1 , Daniela Barile 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2018-05-25 00:00:00 , DOI: 10.1021/acs.jafc.8b01515 Rose M. Nijman 1 , Yan Liu 2 , Apichaya Bunyatratchata 2 , Jennifer T. Smilowitz 2 , Bernd Stahl 1 , Daniela Barile 2
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Oligosaccharides are known to affect the health of infants. The analysis of these complex molecules in (human) milk samples requires state-of-the-art techniques. This study analyzed the composition and concentration of oligosaccharides in early (day 3) and mature (day 42) human milk as well as in five different infant formula brands. The oligosaccharide content decreased in human milk from 9.15 ± 0.25 g/L at day 3 to 6.38 ± 0.29 g/L at day 42 of lactation. All formulas resulted to be fortified with galacto-oligosaccharides, with one also fortified with polydextrose and another with long-chain fructo-oligosaccharides. About 130 unique oligosaccharide structures were identified in the human milk samples, whereas infant formula contained less diversity of structures. The comparisons indicated that composition and abundance of oligosaccharides unique to human milk are not yet reproduced in infant formulas. The analytical workflow developed is suitable for the determination of prebiotic oligosaccharides in foods that contain diverse carbohydrate structures.
中文翻译：
人乳和婴儿配方奶中低聚糖的表征和定量
已知寡糖会影响婴儿的健康。对（人类）牛奶样品中这些复杂分子的分析需要最新技术。这项研究分析了早期（第3天）和成熟（第42天）人乳以及五个不同婴儿配方奶粉品牌中低聚糖的组成和浓度。人乳中的低聚糖含量从哺乳第3天的9.15±0.25 g / L降低到哺乳第42天的6.38±0.29 g / L。结果表明，所有配方均用半乳糖低聚糖强化，其中一种还用聚葡萄糖强化，而另一种则用长链低聚果糖强化。在人乳样品中鉴定出约130种独特的低聚糖结构，而婴儿配方食品的结构多样性较低。比较结果表明，婴儿配方食品中尚未复制出人乳特有的低聚糖的组成和含量。开发的分析工作流程适用于测定包含多种碳水化合物结构的食品中的益生元低聚糖。
更新日期：2018-05-25
中文翻译：
人乳和婴儿配方奶中低聚糖的表征和定量
已知寡糖会影响婴儿的健康。对（人类）牛奶样品中这些复杂分子的分析需要最新技术。这项研究分析了早期（第3天）和成熟（第42天）人乳以及五个不同婴儿配方奶粉品牌中低聚糖的组成和浓度。人乳中的低聚糖含量从哺乳第3天的9.15±0.25 g / L降低到哺乳第42天的6.38±0.29 g / L。结果表明，所有配方均用半乳糖低聚糖强化，其中一种还用聚葡萄糖强化，而另一种则用长链低聚果糖强化。在人乳样品中鉴定出约130种独特的低聚糖结构，而婴儿配方食品的结构多样性较低。比较结果表明，婴儿配方食品中尚未复制出人乳特有的低聚糖的组成和含量。开发的分析工作流程适用于测定包含多种碳水化合物结构的食品中的益生元低聚糖。
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