An effective screening method for inhibitors of NO production in natural products using LC-QTOF MS/MS coupled with a cell-based assay was proposed. The ethyl acetate fraction of Catalpa ovata exhibited a strong inhibitory effect on NO production in lipopolysaccharide-induced BV2 microglia cells. We attempted to identify the active constituents of C. ovata by using LC-QTOF MS/MS coupled with a cell-based assay. Peaks at approximately 14–15 min on the MS chromatogram were estimated to be the bioactive constituents. A new iridoid compound, 6-O-trans-feruloyl-3β-hydroxy-7-deoxyrehamaglutin A (4), and nine known compounds (1–3, 5–10) were isolated from the ethyl acetate fraction of C. ovata by repeated column chromatography. Compounds 3, 4, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells. Our results indicate that LC-QTOF MS/MS coupled with a cell-based NO production inhibitory assay successfully predicted active compounds without a time-consuming isolation process.

提出了一种使用LC-QTOF MS / MS结合基于细胞的测定法对天然产物中NO生成抑制剂进行有效筛选的方法。al木的乙酸乙酯级分对脂多糖诱导的BV2小胶质细胞产生NO产生强烈的抑制作用。我们试图通过使用LC-QTOF MS / MS结合基于细胞的测定法来鉴定卵形衣原体的活性成分。MS色谱图上大约14-15分钟的峰被认为是生物活性成分。一个新的环烯醚萜化合物，6- O型反-feruloyl-3β羟基-7- deoxyrehamaglutin A（4），和九已知化合物（1 - 3，5 - 10通过重复柱色谱法从卵形衣原体的乙酸乙酯级分中分离出1）。化合物3，4，5，7，和8显著衰减脂多糖刺激的NO产生中BV2细胞。我们的结果表明，LC-QTOF MS / MS结合基于细胞的NO产生抑制分析可成功预测活性化合物，而无需耗时的分离过程。